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Read our guarantee »Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Anti-EGFR (phospho S1070 + S1071) antibody
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Rabbit polyclonal to EGFR (phospho S1070 + S1071)
ab51108 detects endogenous levels of EGFR only when phosphorylated at serines 1070/1071.
ELISA, IHC-Pmore details
Reacts with
Mouse, Rat, Human
A synthetic phospho-peptide derived from human EGFR around the phosphorylation site of serine 1070/1071 (R-Y-SP-SP-D).
Human breast carcinoma tissue
Liquid
Store at -20°C. Stable for 12 months at -20°C
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab51108 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Polyclonal
IgG
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Immunohistochemistry (Paraffin-embedded sections) - EGFR (phospho S1070 + S1071) antibody (ab51108)
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Our Abpromise guarantee covers the use of ab51108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.
Target information above from: UniProt accessionP00533
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry (Paraffin-embedded sections) - EGFR (phospho S1070 + S1071) antibody (ab51108)

ab51108 (1/100) staining EGFR (phospho S1070 + S1071) in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Right panel represents a negative control experiment done in the presence of the immunizing (blocking) peptide.
ab51108 has not yet been referenced specifically in any publications.
Publishing research using ab51108? Please let us know so that we can cite the reference in this datasheet
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ab51108 (1/100) staining EGFR (phospho S1070 + S1071) in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Right panel represents a negative control experiment done in the presence of the immunizing (blocking) peptide.
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