Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-EGFR (phospho S1070 + S1071) antibody
See all EGFR products (87) ...
Description
Rabbit polyclonal to EGFR (phospho S1070 + S1071)
Specificity
ab51108 detects endogenous levels of EGFR only when phosphorylated at serines 1070/1071.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic phospho-peptide derived from human EGFR around the phosphorylation site of serine 1070/1071 (R-Y-SP-SP-D).
Positive control
Human breast carcinoma tissue
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab51108 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
-
Immunohistochemistry (Paraffin-embedded sections) - EGFR (phospho S1070 + S1071) antibody (ab51108)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
Target
Function
Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
Tissue specificity
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Involvement in disease
Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
Cellular localization
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.
Target information above from: UniProt accessionP00533
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EGFR antibody
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Cell growth inhibiting protein 40 antibody
see all
Database links
- Entrez Gene: 1956 Human
- Entrez Gene: 13649 Mouse
- Entrez Gene: 24329 Rat
- Omim: 131550 Human
- SwissProt: P00533 Human
- SwissProt: Q01279 Mouse
- Unigene: 488293 Human
- Unigene: 420648 Mouse
see all
Anti-EGFR (phospho S1070 + S1071) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - EGFR (phospho S1070 + S1071) antibody (ab51108)
ab51108 (1/100) staining EGFR (phospho S1070 + S1071) in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Right panel represents a negative control experiment done in the presence of the immunizing (blocking) peptide.
References for Anti-EGFR (phospho S1070 + S1071) antibody (ab51108)
ab51108 has not yet been referenced specifically in any publications.
Publishing research using ab51108? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"