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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab38503 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Paraffin-embedded sections) - EGFR (phospho S1070) antibody (ab38503)
Ab38503, at a 1/50 dilution, staining EGFR in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: untreated.
Right image: treated with synthesized phosphopeptide
Western blot - EGFR (phospho S1070) antibody (ab38503)
All lanes : Anti-EGFR (phospho S1070) antibody (ab38503) at 1/500 dilution
Lane 1 : SK-OV3 cell extract
Lane 2 : SK-OV3 cell extract treated with EGF
Lysates/proteins at 30 µg per lane.
Secondary
Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size : 134 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
The amount of cell extract loaded for the WB was 5-30 ug of total protein.
The 160 kDa protein band shown in the Western blot image is the glycosylated form of EGFR.
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