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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Overview
Product name
Anti-EGFR (phospho S1070) antibody
See all EGFR products (87) ...
Description
Rabbit polyclonal to EGFR (phospho S1070)
Specificity
This antibody is specific for EGFR only when phosphorylated at serine 1070.
Tested applications
WB, IHC-P, ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from Human EGFR around the phosphorylation site of serine 1070.
Positive control
WB: SK-OV3 cell extract. IHC-P: Human breast carcinoma tissue slides.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Signal transduction >> Protein phosphorylation >> Tyrosine kinases >> Receptor tyrosine kinases
Signal Transduction >> Growth Factors/Hormones >> EGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 160 kDa (predicted molecular weight: 134 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/20000ELISA: 1/20000
IP: Use at an assay dependent dilution.
Target
Function
Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
Tissue specificity
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
Involvement in disease
Defects in EGFR are associated with lung cancer (LNCR) [MIM:211980].
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.
Cellular localization
Secreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Co-localizes with TNK2 on the endosomes.
Target information above from: UniProt accessionP00533
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EGFR antibody
- Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
- Avian erythroblastic leukemia viral (verbb) oncogene homolog antibody
see all
Database links
- Entrez Gene: 1956 Human
- Entrez Gene: 13649 Mouse
- Entrez Gene: 24329 Rat
- Omim: 131550 Human
- SwissProt: P00533 Human
- SwissProt: Q01279 Mouse
- Unigene: 488293 Human
- Unigene: 420648 Mouse
see all
Anti-EGFR (phospho S1070) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - EGFR (phospho S1070) antibody (ab38503)
Ab38503, at a 1/50 dilution, staining EGFR in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: untreated.
Right image: treated with synthesized phosphopeptide
Western blot - EGFR (phospho S1070) antibody (ab38503)
All lanes : Anti-EGFR (phospho S1070) antibody (ab38503) at 1/500 dilution
Lane 1 : SK-OV3 cell extract
Lane 2 : SK-OV3 cell extract treated with EGF
Lysates/proteins at 30 µg per lane.
Secondary
Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size : 134 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
The amount of cell extract loaded for the WB was 5-30 ug of total protein.
The 160 kDa protein band shown in the Western blot image is the glycosylated form of EGFR.
References for Anti-EGFR (phospho S1070) antibody (ab38503)
ab38503 has not yet been referenced specifically in any publications.
Publishing research using ab38503? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"