Anti-EGFR (phospho S1142) antibody (ab76194)

Overview

  • Product nameAnti-EGFR (phospho S1142) antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR (phospho S1142)
  • Specificityab76194 is not predicted to react with other ErbB family members.
  • Tested applicationsWB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human EGFR conjugated to Keyhole Limpet Haemocyanin (KLH). The aa numbering is around serine 1166 if the signal peptide is included.
    Database link: P00533

  • Positive control
    • IHC-P: A431 cells untreated or treated with Calyculin A (50 nM) for 15 minutes. WB: A431 cell lystes treated with Calyculin A (100 nM) for 30 minutes.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 134 kDa.
ELISA 1/2000.
ICC/IF 1/200.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR (phospho S1142) antibody images

  • ab76194, at a 1/200 dilution, staining EGFR in A431 cells by Immunoflurescence.
    Left image: untreated.
    Middle image: treated with Calyculin A (50 nM) for 15 minutes.
    Right image: treated with Calyculin A (50 nM) for 15 minutes with blocking peptide, phospho EGFR (Ser-1142).
  • All lanes : Anti-EGFR (phospho S1142) antibody (ab76194) at 1/1000 dilution

    Lane 1 : A431 cells treated with Calyculin A (100 nM) for 30 minutes
    Lane 2 : A431 cells treated with Calyculin A (100 nM) for 30 minutes and then treated with lambda phosphatase


    Predicted band size : 134 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)

References for Anti-EGFR (phospho S1142) antibody (ab76194)

ab76194 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your recent telephone enquiry which I have investigated further. As discussed on the telephone, there is a lot of post translational modification on this protein according to SwissProt/Uniprot site, which indicates that its weight on ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"