Anti-EGFR (phospho S695) antibody [EPR2257] (ab92629)

Overview

• Product nameAnti-EGFR (phospho S695) antibody [EPR2257]See all EGFR primary antibodies ...
• Description
  Rabbit monoclonal [EPR2257] to EGFR (phospho S695)
• SpecificityDetects EGFR when phosphorylated on serine 695 numbering based on full length EGFR including 24aa Signal pepidase which cleaved to produce mature EGFR then Ser671 is phosphorylated
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues surrounding Serine 695 in Human EGFR

• Positive controlA431 cell lysate treated with EGF
• General notesProduced under U.S Patent No. 5,675,063

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR2257
• IsotypeIgG
• Research Areas
  • Cancer
  • Tumor biomarkers
  • Receptors

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Tyrosine kinases
  • Receptor tyrosine kinases

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • EGF

Applications

Target

• FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
  Isoform 2 may act as an antagonist of EGF action.
• Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
• Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
  Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
  Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
• Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.

Target information above from: UniProt accession P00533 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1956 Human
  • Omim: 131550 Human
  • SwissProt: P00533 Human
  • Unigene: 488293 Human

• Alternative names
  Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibodyCell growth inhibiting protein 40 antibodyCell proliferation inducing protein 61 antibody

  EGF R antibodyEGFR antibodyEGFR_HUMAN antibodyEpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibodyEpidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibodyEpidermal growth factor receptor antibodyErbb antibodyErbb1 antibodyERBB1 antibodyErrp antibodyHER1 antibodymENA antibodyPIG61 antibodyProto-oncogene c-ErbB-1 antibodyReceptor tyrosine protein kinase ErbB 1 antibodyReceptor tyrosine-protein kinase ErbB-1 antibodywa2 antibodyWa5 antibody

  see all

Anti-EGFR (phospho S695) antibody [EPR2257] images

• 

  Western blot - EGFR (phospho S695) antibody [EPR2257] (ab92629)
  All lanes : Anti-EGFR (phospho S695) antibody [EPR2257] (ab92629) at 1/1000 dilution
  
  Lane 1 : A431 cell lysate untreated
  Lane 2 : A431 cell lysate treated with EGF
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 134 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - EGFR (phospho S695) antibody [EPR2257] (ab92629)
  Immunofluorescent staining of A431 cells using ab92629 at 1/100 dilution.