Anti-EGFR (phospho S695) antibody [EPR2257] (ab92629)

Overview

  • Product nameAnti-EGFR (phospho S695) antibody [EPR2257]
    See all EGFR primary antibodies
  • Description
    Rabbit monoclonal [EPR2257] to EGFR (phospho S695)
  • SpecificityDetects EGFR when phosphorylated on serine 695 numbering based on full length EGFR including 24aa Signal pepidase which cleaved to produce mature EGFR then Ser671 is phosphorylated
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding Serine 695 in Human EGFR

  • Positive control
    • A431 cell lysate treated with EGF
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: 1/100 - 1/250.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 134 kDa.

    Is unsuitable for Flow Cyt, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho S695) antibody [EPR2257] images

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: EGFR (pS695) phospho peptide

      Lane 2 sample: EGFR non-phospho peptide

      Exposure time: 3 minutes

    • All lanes : Anti-EGFR (phospho S695) antibody [EPR2257] (ab92629) at 1/1000 dilution

      Lane 1 : A431 cell lysate untreated
      Lane 2 : A431 cell lysate treated with EGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 134 kDa
    • Immunofluorescent staining of A431 cells using ab92629 at 1/100 dilution.

    References for Anti-EGFR (phospho S695) antibody [EPR2257] (ab92629)

    ab92629 has not yet been referenced specifically in any publications.

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"