Anti-EGFR (phospho T654) antibody [3F2] (ab78283)

Overview

  • Product nameAnti-EGFR (phospho T654) antibody [3F2]
    See all EGFR primary antibodies
  • Description
    Mouse monoclonal [3F2] to EGFR (phospho T654)
  • Tested applicationsWB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    KLH conjugated synthetic peptide encompassing the amino acids surrounding Thr 654 in human EGFR

  • Positive control
    • PMA and pervanadate treated Hep2G cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab78283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
  • Application notesELISA: Use at a concentration of 0.05 µg/ml.
    IP: Use 1-10µg for 10 cells treated with vanadate.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 134 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho T654) antibody [3F2] images

    • Anti-EGFR (phospho T654) antibody [3F2] (ab78283) at 1 µg/ml + Lysates from PMA and pervanadate treated HepG2 cells

      Secondary
      goat anti mouse secondary antibody conjugated to HRP at 1/5000 dilution
      developed using the ECL technique

      Predicted band size : 134 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)

    References for Anti-EGFR (phospho T654) antibody [3F2] (ab78283)

    ab78283 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab78283.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"