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Anti-EGFR (phospho T654) antibody [3F2] (ab78283)

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Overview

  • Product nameAnti-EGFR (phospho T654) antibody [3F2]See all EGFR primary antibodies ...
  • Description
    Mouse monoclonal [3F2] to EGFR (phospho T654)
  • Tested applicationsWB, IP, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    KLH conjugated synthetic peptide encompassing the amino acids surrounding Thr 654 in human EGFR

  • Positive controlPMA and pervanadate treated Hep2G cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab78283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
ELISA
  • Application notesELISA: Use at a concentration of 0.05 µg/ml.
    IP: Use 1-10µg for 10 cells treated with vanadate.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 134 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
      Isoform 2 may act as an antagonist of EGF action.
    • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
    • Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications      Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
      Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
      Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
    • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.

      • Target information above from: UniProt accession P00533 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibodyCell growth inhibiting protein 40 antibodyCell proliferation inducing protein 61 antibody
        EGF R antibodyEGFR antibodyEGFR_HUMAN antibodyEpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibodyEpidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibodyEpidermal growth factor receptor antibodyerbb 1 antibodyErbb antibodyErbb1 antibodyERBB1 antibodyErrp antibodyHER1 antibodymENA antibodyOncogene ERBB antibodyPIG61 antibodyProto-oncogene c-ErbB-1 antibodyReceptor tyrosine protein kinase ErbB 1 antibodyReceptor tyrosine-protein kinase ErbB-1 antibodyUrogastrone antibodywa2 antibodyWa5 antibody
      see all

    Anti-EGFR (phospho T654) antibody [3F2] images

    • Western blot - EGFR (phospho T654) antibody [3F2] (ab78283)
      Western blot - EGFR (phospho T654) antibody [3F2] (ab78283)
      Anti-EGFR (phospho T654) antibody [3F2] (ab78283) at 1 µg/ml + Lysates from PMA and pervanadate treated HepG2 cells

      Secondary
      goat anti mouse secondary antibody conjugated to HRP at 1/5000 dilution
      developed using the ECL technique

      Predicted band size : 134 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)

    References for Anti-EGFR (phospho T654) antibody [3F2] (ab78283)

    ab78283 has not yet been referenced specifically in any publications.

    Publishing research using ab78283 ? Please let us know so that we can cite the reference in this datasheet

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