Anti-EGFR (phospho T678) antibody [EP2255Y] (ab75986)

Overview

  • Product nameAnti-EGFR (phospho T678) antibody [EP2255Y]
    See all EGFR primary antibodies
  • Description
    Rabbit monoclonal [EP2255Y] to EGFR (phospho T678)
  • SpecificityDetects EGFR when phosphorylated on threonine 678 numbering based on full length EGFR including 24aa Signal pepidase which cleaved to produce mature EGFR then T654 is phosphorylated
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding threonine 678 of human EGFR

  • Positive control
    • ICC/IF: A431 cells WB: A431 cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/20000 - 1/50000. Predicted molecular weight: 134 kDa.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho T678) antibody [EP2255Y] images

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: EGFR (pT678) phospho peptide

      Lane 2 sample: EGFR non-phospho peptide

      Exposure time: 3 minutes

    • All lanes : Anti-EGFR (phospho T678) antibody [EP2255Y] (ab75986) at 1/50000 dilution

      Lane 1 : A431 (human epidermoid carcinoma) treated with Epidermal Growth Factor (EGF) whole cell lysates
      Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Predicted band size : 134 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Blocking and dilution buffer: 5% NFDM /TBST

    • All lanes : Anti-EGFR (phospho T678) antibody [EP2255Y] (ab75986) at 1/50000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : A431 cell lysate treated with EGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 134 kDa
      Observed band size : 175 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of EGFR (phospho T678) in A431 cells, using ab75986 at a 1/100 dilution.

    References for Anti-EGFR (phospho T678) antibody [EP2255Y] (ab75986)

    ab75986 has not yet been referenced specifically in any publications.

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