Overview

  • Product nameAnti-EGFR (phospho T693) antibody [EP2256Y]
    See all EGFR primary antibodies
  • Description
    Rabbit monoclonal [EP2256Y] to EGFR (phospho T693)
  • SpecificityDetects EGFR when phosphorylated on threonine 693 numbering based on full length EGFR including 24aa Signal pepidase which cleaved to produce mature EGFR then T669 is phosphorylated
  • Tested applicationsICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 693 of human EGFR

  • Positive control
    • A431 cell lysates, untreated or treated with EGF; A431 cells.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/20000. Detects a band of approximately 175 kDa (predicted molecular weight: 134 kDa).
IP 1/40.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho T693) antibody [EP2256Y] images

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: EGFR (pT693) phospho peptide

      Lane 2 sample: EGFR non-phospho peptide

      Exposure time: 3 minutes

    • All lanes : Anti-EGFR (phospho T693) antibody [EP2256Y] (ab75980) at 1/20000 dilution

      Lane 1 : A431 (human epidermoid carcinoma) treated with Epidermal Growth Factor (EGF) whole cell lysates
      Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Predicted band size : 134 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-EGFR (phospho T693) antibody [EP2256Y] (ab75980) at 1/20000 dilution

      Lane 1 : A431 cell lysate, untreated
      Lane 2 : A431 cell lysate, treated with EGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 134 kDa
      Observed band size : 175 kDa (why is the actual band size different from the predicted?)
    • ab75980 at 1/100 dilution staining EGFR in A431 cells by Immunofluorescence.

    References for Anti-EGFR (phospho T693) antibody [EP2256Y] (ab75980)

    This product has been referenced in:
    • Johansson M  et al. The soluble form of the tumor suppressor Lrig1 potently inhibits in vivo glioma growth irrespective of EGF receptor status. Neuro Oncol 15:1200-11 (2013). WB ; Human . Read more (PubMed: 23723255) »
    • Ververis K & Karagiannis TC An atlas of histone deacetylase expression in breast cancer: fluorescence methodology for comparative semi-quantitative analysis. Am J Transl Res 4:24-43 (2012). ICC/IF ; Human . Read more (PubMed: 22347520) »

    See all 2 Publications for this product

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