Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928)

Overview

  • Product nameAnti-EGFR (phospho Y1045) antibody [11C2]
    See all EGFR primary antibodies
  • Description
    Mouse monoclonal [11C2] to EGFR (phospho Y1045)
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Phosphopeptide conjugate

  • General notes


    The phosphorylation status of Tyrosine 1045 regulates the degradation of activated EGFR. Autophosphorylation of Tyrosine 1045 creates a docking site for the c-cbl ubiquitination ligase.

Properties

Applications

Our Abpromise guarantee covers the use of ab24928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.05 µg/ml.
WB Use a concentration of 0.01 µg/ml. Predicted molecular weight: 134 kDa.

Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. Dilution optimised using Chromogenic detection.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR (phospho Y1045) antibody [11C2] images

  • All lanes : Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928) at 0.5 µg/ml (EGFR Activation: Serum starved MDA-MB 468 cells were incubated with 10ng/ml EGF for the indicated times. Whole cell lysates were prepared and separated by SDS-PAGE)

    Lane 1 : control
    Lane 2 : 5 min EGF
    Lane 3 : 15 min EGF
    Lane 4 : 30 min EGF
    Lane 5 : 1 hour EGF
    Lane 6 : 2 hours EGF
    Lane 7 : 4 hours EGF
    Lane 8 : 8 hours EGF

    Lysates/proteins at 20000 cells per lane.

    Secondary
    A HRP-conjugated antibody
    developed using the ECL technique

    Predicted band size : 134 kDa


    Exposure time : 30 seconds

References for Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928)

ab24928 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab24928.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"