Purification notesThiophilic adsorption and size exclusion chromatography.
Primary antibody notesThe phosphorylation status of Tyrosine 1045 regulates the degradation of activated EGFR. Autophosphorylation of Tyrosine 1045 creates a docking site for the c-cbl ubiquitination ligase.
Western blot - EGFR (phospho Y1045) antibody [11C2] (ab24928)
All lanes : Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928) at 0.5 µg/ml (EGFR Activation:
Serum starved MDA-MB 468 cells were incubated with 10ng/ml EGF for the indicated times. Whole cell lysates were prepared and separated by SDS-PAGE)
Lane 1 : control Lane 2 : 5 min EGF Lane 3 : 15 min EGF Lane 4 : 30 min EGF Lane 5 : 1 hour EGF Lane 6 : 2 hours EGF Lane 7 : 4 hours EGF Lane 8 : 8 hours EGF
Lysates/proteins at 20000 cells per lane.
Secondary A HRP-conjugated antibody developed using the ECL technique
Predicted band size : 134 kDa
Exposure time : 30 seconds
References for Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928)
has not yet been referenced specifically in any publications.
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