Anti-EGFR (phospho Y1068) antibody (ab92960)

Overview

  • Product nameAnti-EGFR (phospho Y1068) antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR (phospho Y1068)
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 - 1150 of Human EGFR, phosphorylated at Y1068.

  • Positive control
    • This antibody gave a positive signal in A431 EGF treated whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab92960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 134 kDa).

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR (phospho Y1068) antibody images

  • All lanes : Anti-EGFR (phospho Y1068) antibody (ab92960) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole cell Lysate-TNFa Stimulates Whole Cell Lysate (ab94364)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole cell Lysate-TNFa Stimulates Whole Cell Lysate (ab94364) with Immunizing peptide at 1 µg/ml
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole cell Lysate-TNFa Stimulates Whole Cell Lysate (ab94364) with Non-modified peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-EGFR (phospho Y1068) antibody (ab92960)

ab92960 has not yet been referenced specifically in any publications.

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