Overview

  • Product nameAnti-EGFR (phospho Y1086) antibody [Y39]
    See all EGFR primary antibodies
  • Description
    Rabbit monoclonal [Y39] to EGFR (phospho Y1086)
  • SpecificityThis antibody only detects EGFR phosphorylated on Tyrosine 1086 of the mature human isoform 1.
  • Tested applicationsWB, ICC, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho peptide corresponding to residues surrounding Tyr 1086 of human EGFR.

  • Positive control
    • A431 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 170 kDa (predicted molecular weight: 134 kDa).
ICC 1/100 - 1/250.
IP 1/150.
  • Application notesIs unsuitable for Flow Cyt or IHC.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho Y1086) antibody [Y39] images

    • All lanes : Anti-EGFR (phospho Y1086) antibody [Y39] (ab32086) at 1/10000 dilution

      Lane 1 : Untreated A431 cell lysate
      Lane 2 : A431 cell lysate treated with EGF


      Predicted band size : 134 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent analysis of EGFR (phospho Y1086) expression in A431 cell culture, using 1/100 ab32086.

    References for Anti-EGFR (phospho Y1086) antibody [Y39] (ab32086)

    This product has been referenced in:
    • Johansson M  et al. The soluble form of the tumor suppressor Lrig1 potently inhibits in vivo glioma growth irrespective of EGF receptor status. Neuro Oncol 15:1200-11 (2013). WB ; Human . Read more (PubMed: 23723255) »
    • Xu ZH  et al. RAF1-MEK1-ERK/AKT axis may confer NSCLC cell lines resistance to erlotinib. Int J Clin Exp Pathol 6:1493-504 (2013). WB ; Human . Read more (PubMed: 23923067) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"