Anti-EGFR (phospho Y1148) antibody (ab5651)

Overview

  • Product nameAnti-EGFR (phospho Y1148) antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR (phospho Y1148)
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide (Human) derived from the region of EGFR that contains tyrosine 1148.

  • Positive control
    • A431 cells stimulated with EGF and/or NIH3T3 cells expressing human EGFR and stimulated with EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab5651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 140 kDa.

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR (phospho Y1148) antibody images

  • Cell extracts prepared from NIH3T3 cells expressing EGFR and either not stimulated (-) or stimulated (+) with EGF were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were then transferred to nitrocellulose and incubated with 0.50 µg/mL ab5651 antibody, following prior incubation with: no peptide (=None), the non-phosphorylated peptide corresponding to the phosphopeptide (=EGFR [pY1148] NP), or the phosphopeptide immunogen (=EGFR [pY1148] P). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase conjugate and bands were detected using the Tropix WesternStar method. The data presented here show that only the phosphopeptide corresponding to this site completely blocks the antibody signal, demonstrating the high specificity of the ab5651 antibody for this phosphorylated residue.

References for Anti-EGFR (phospho Y1148) antibody (ab5651)

ab5651 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and your interest in our products. According to Swiss-Prot database the expected molecular weight of Receptor tyrosine-protein kinase ErbB-1 is 134,200 kDa. Looking at the sequence of the aminoacide and features of th...

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