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Phosphopeptide conjugate (Human).
Our Abpromise guarantee covers the use of ab24912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 134 kDa.Can be blocked with EGFR (phospho Y1173) peptide (ab87004).|
|IP||Use 1-10µg for 106 cells.|
|ELISA||Use a concentration of 0.05 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20575069|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"