Anti-EGFR (phospho Y1173) antibody (ab5652)

Overview

  • Product nameAnti-EGFR (phospho Y1173) antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR (phospho Y1173)
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic phosphopeptide (Human) derived from the region of EGFR that contains tyrosine 1173.

  • Positive control
    • NIH3T3 cells expressing human EGFR. Human epidermoid carcinoma (A431) cells +/- EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab5652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Suggested working dilution of 1/100 (see PMID: 17123512).
    WB: 1/1000. Detects a band of approximately 185 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho Y1173) antibody images

    • Cell extracts prepared from NIH3T3 cells expressing EGFR were starved for 30 hours, then stimulated for 10 minutes with 30 ng/mL EGF (+), or left unstimulated (-), then resolved by SDS-PAGE on a 6% Tris-glycine gel, and transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL ab5652 antibody, following prior incubation in the absence (lanes 1& 2), or presence of the peptide immunogen (lanes 3 & 4), or the nonphosphopeptide corresponding to the EGFR phosphopeptide (lanes 5 & 6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of the ab5652 antibody for this phosphorylated residue.

    References for Anti-EGFR (phospho Y1173) antibody (ab5652)

    This product has been referenced in:
    • Doan PL  et al. Epidermal growth factor regulates hematopoietic regeneration after radiation injury. Nat Med 19:295-304 (2013). Mouse . Read more (PubMed: 23377280) »
    • Bade LK  et al. Mammary tumorigenesis induced by fibroblast growth factor receptor 1 requires activation of the epidermal growth factor receptor. J Cell Sci 124:3106-17 (2011). WB ; Mouse . Read more (PubMed: 21868365) »

    See all 4 Publications for this product

    Product Wall

    Thank you very much for your call last week and for your patience while I've been in touch with the production lab about this image.

    The lab has confirmed that human EGF was used to treat the cells that were used in the Western blot testing. ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (CGN)
    Loading amount 20 µg
    Specification CGN
    Treatment 100 ug Fibrinogen for 20 hrs
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Username

    Abcam user community

    Verified customer

    Submitted Dec 11 2006

    We have already arranged a refund for your. However, we would like to let you know that we have just received the results from the lab. We have retested the same batch you received, the protein content of this lot is 5.10 mg/ml. We assay the protei...

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    Thank you for your patience. Regarding ab5636, the antibody has only been tested so far on cells. There was a mistake on the datasheet which I have since updated. I apologize for the inconvenience and if you have any more questions, please contact us a...

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    Thank you for your enquiry. Ab5652 has only been tested on frozen sections. I will contact you again once I have heard back from the originator of ab5636.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"