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Anti-EGFR (phospho Y1173) antibody (ab84470)

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Overview

  • Product nameAnti-EGFR (phospho Y1173) antibodySee all EGFR primary antibodies ...
  • Description
    Rabbit polyclonal to EGFR (phospho Y1173)
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Human EGFR, phosphorylated at Y1173.

    Read Abcam's proprietary immunogen policy

  • Positive controlThis antibody gave a positive signal in EGF-Treated A431 Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 134 kDa).

Target

  • FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
    Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
  • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.

    • Target information above from: UniProt accession P00533 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibodyCell growth inhibiting protein 40 antibodyCell proliferation inducing protein 61 antibody
      EGF R antibodyEGFR antibodyEGFR_HUMAN antibodyEpidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibodyEpidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibodyEpidermal growth factor receptor antibodyErbb antibodyErbb1 antibodyERBB1 antibodyErrp antibodyHER1 antibodymENA antibodyPIG61 antibodyProto-oncogene c-ErbB-1 antibodyReceptor tyrosine protein kinase ErbB 1 antibodyReceptor tyrosine-protein kinase ErbB-1 antibodywa2 antibodyWa5 antibody
    see all

Anti-EGFR (phospho Y1173) antibody images

  • Western blot - EGFR (phospho Y1173) antibody (ab84470)
    Western blot - EGFR (phospho Y1173) antibody (ab84470)
    All lanes : Anti-EGFR (phospho Y1173) antibody (ab84470) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : EGF-Treated A431 Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 90 seconds
  • Western blot - EGFR (phospho Y1173) antibody (ab84470)
    Western blot - EGFR (phospho Y1173) antibody (ab84470)
    All lanes : Anti-EGFR (phospho Y1173) antibody (ab84470) at 1 µg/ml

    Lane 1 : EGF-Treated A431 Whole Cell Lysate
    Lane 2 : EGF-Treated A431 Whole Cell Lysate with Immunising Peptide at 1 µg/ml
    Lane 3 : EGF-Treated A431 Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References for Anti-EGFR (phospho Y1173) antibody (ab84470)

ab84470 has not yet been referenced specifically in any publications.

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