Anti-EGFR (phospho Y1173) antibody (ab84470)

Overview

  • Product nameAnti-EGFR (phospho Y1173) antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR (phospho Y1173)
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human EGFR aa 1150 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab104700)

  • Positive control
    • This antibody gave a positive signal in EGF-Treated A431 Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 134 kDa).

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • erb-b2 receptor tyrosine kinase 1 antibody
    • ERBB antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • NISBD2 antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • Urogastrone antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR (phospho Y1173) antibody images

  • All lanes : Anti-EGFR (phospho Y1173) antibody (ab84470) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : EGF-Treated A431 Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 90 seconds
  • All lanes : Anti-EGFR (phospho Y1173) antibody (ab84470) at 1 µg/ml

    Lane 1 : EGF-Treated A431 Whole Cell Lysate
    Lane 2 : EGF-Treated A431 Whole Cell Lysate with Immunising Peptide at 1 µg/ml
    Lane 3 : EGF-Treated A431 Whole Cell Lysate with Non-Modified Control Peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References for Anti-EGFR (phospho Y1173) antibody (ab84470)

ab84470 has not yet been referenced specifically in any publications.

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