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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration information loading...
Purity Immunogen affinity purified
Purification notes The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/4000 - 1/20000.
1/250 - 1/500. Predicted molecular weight: 170 kDa.
Information by UniProt
Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
Cell growth inhibiting protein 40 antibody
Cell proliferation inducing protein 61 antibody
Anti-EGFR (phospho Y1197) antibody images
Western blot - EGFR (phospho Y1197) antibody (ab34901)
All lanes : Anti-EGFR (phospho Y1197) antibody (ab34901) at 2.2 µg/ml Lane 1 : A431 whole cell lysate Lane 2 : A431 whole cell lysate, stimulated with EGF (50 ng/ml for 15 min) Lysates/proteins at 30 µg per lane. Secondary Near infrared fluorescent dye conjugated Goat anti-Rabbit IgG Predicted band size : 170 kDa Observed band size : 170 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EGFR (phospho Y1197) antibody (ab34901)
ab34901 at 5 µg/ml staining placental trophoblasts at 40X. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
References for Anti-EGFR (phospho Y1197) antibody (ab34901)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"