Overview

  • Product nameAnti-EGFR (phospho Y845) antibody
    See all EGFR primary antibodies
  • Description
    Rabbit polyclonal to EGFR (phospho Y845)
  • Tested applicationsICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 845.

  • Positive control
    • Human Epidermoid Carcinoma (A431) cells and cortic smooth muscle cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ICC
  • Application notesICC: Use at an assay dependent dilution.
    ICC/IF: 1/50 (PMID 19147829).
    WB: Use at a concentration of 0.1 - 1.0 µg/ml. Detects a band of approximately 185 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
      • Cell growth inhibiting protein 40 antibody
      • Cell proliferation inducing protein 61 antibody
      • EGF R antibody
      • EGFR antibody
      • EGFR_HUMAN antibody
      • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
      • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
      • Epidermal growth factor receptor antibody
      • erb-b2 receptor tyrosine kinase 1 antibody
      • ERBB antibody
      • ERBB1 antibody
      • Errp antibody
      • HER1 antibody
      • mENA antibody
      • NISBD2 antibody
      • Oncogen ERBB antibody
      • PIG61 antibody
      • Proto-oncogene c-ErbB-1 antibody
      • Receptor tyrosine protein kinase ErbB 1 antibody
      • Receptor tyrosine-protein kinase ErbB-1 antibody
      • SA7 antibody
      • Species antigen 7 antibody
      • Urogastrone antibody
      • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
      • wa2 antibody
      • Wa5 antibody
      see all

    Anti-EGFR (phospho Y845) antibody images

    • Peptide Competition: Extracts prepared from A431 cells stimulated with EGF were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA TBST buffer overnight at 4°C, then incubated with 0.40 µg/mL ab5636 antibody, for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: no peptide (1), the phosphopeptide immunogen (2), the non-phosphopeptide corresponding to the immunogen (3), or a generic phosphotyrosine containing peptide (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to ab5636 blocks the antibody signal, demonstrating the specificity of the antibody.
    • Immunodetection using ab5636 in cultured cortic smooth muscle cells treated with Angiotensin II.

    References for Anti-EGFR (phospho Y845) antibody (ab5636)

    This product has been referenced in:
    • Jaldety Y  et al. Sperm EGFR mediates a7 AChR activation to promote fertilization. J Biol Chem : (2012). Read more (PubMed: 22577141) »
    • Pontillo CA  et al. Activation of c-Src/HER1/STAT5b and HER1/ERK1/2 signaling pathways and cell migration by hexachlorobenzene in MDA-MB-231 human breast cancer cell line. Toxicol Sci 120:284-96 (2011). Read more (PubMed: 21205633) »

    See all 4 Publications for this product

    Product Wall

    We have already arranged a refund for your. However, we would like to let you know that we have just received the results from the lab. We have retested the same batch you received, the protein content of this lot is 5.10 mg/ml. We assay the protei...

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    Thank you again for your patience. Regarding ab30, EGFR1 recognises the whole receptor protein rather than a truncated version. For ab5636, the synthetic peptide immunogen to produce the antibody is approximately 10 amino acid residues around EGFR [pY8...

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    Thank you for your patience. I have received the following information thus far: Ab231: In Western blotting ab231 detects a 170kDa band which corresponds to EGFR. The epitope recognized by this antibody is located on the extracellular domain of the...

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    Thank you for your patience. Regarding ab5636, the antibody has only been tested so far on cells. There was a mistake on the datasheet which I have since updated. I apologize for the inconvenience and if you have any more questions, please contact us a...

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    Thank you for your enquiry. Ab5652 has only been tested on frozen sections. I will contact you again once I have heard back from the originator of ab5636.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"