Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)

Overview

  • Product nameAnti-EGFR (phospho Y992) antibody [EM-12]
    See all EGFR primary antibodies
  • Description
    Mouse monoclonal [EM-12] to EGFR (phospho Y992)
  • Specificityab81440 recognises EGFR phosphorylated on tyrosine 992.
  • Tested applicationsFlow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phospho-peptide covering sequence around tyrosine 992 of Human EGFR.

  • Positive control
    • EGF-stimulated A431. A431, CALU-3, Jurkat cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab81440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 134 kDa).
IP Use at an assay dependent dilution.

Target

  • FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
    Isoform 2 may act as an antagonist of EGF action.
  • Tissue specificityUbiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
  • Involvement in diseaseDefects in EGFR are associated with lung cancer (LNCR) [MIM:211980]. LNCR is a common malignancy affecting tissues of the lung. The most common form of lung cancer is non-small cell lung cancer (NSCLC) that can be divided into 3 major histologic subtypes: squamous cell carcinoma, adenocarcinoma, and large cell lung cancer. NSCLC is often diagnosed at an advanced stage and has a poor prognosis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3.
    Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur. Deubiquitinated by OTUD7B, preventing degradation.
    Methylated. Methylation at Arg-1199 by PRMT5 positively stimulates phosphorylation at Tyr-1197.
  • Cellular localizationSecreted and Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. Nucleus membrane. Endosome. Endosome membrane. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    • Cell growth inhibiting protein 40 antibody
    • Cell proliferation inducing protein 61 antibody
    • EGF R antibody
    • EGFR antibody
    • EGFR_HUMAN antibody
    • Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    • Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    • Epidermal growth factor receptor antibody
    • Erbb antibody
    • Erbb1 antibody
    • ERBB1 antibody
    • Errp antibody
    • HER1 antibody
    • mENA antibody
    • Oncogen ERBB antibody
    • PIG61 antibody
    • Proto-oncogene c-ErbB-1 antibody
    • Receptor tyrosine protein kinase ErbB 1 antibody
    • Receptor tyrosine-protein kinase ErbB-1 antibody
    • SA7 antibody
    • Species antigen 7 antibody
    • v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    • wa2 antibody
    • Wa5 antibody
    see all

Anti-EGFR (phospho Y992) antibody [EM-12] images

  • All lanes : Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440) at 1 µg/ml

    Lane 1 : EGF-treated A431 cell line
    Lane 2 : EGF-treated CALU-3 cell line
    Lane 3 : EGF-treated MCF-7 cell line
    Lane 4 : EGF-treated Jurkat cell line
    Lane 5 : EGF-treated Ramos cell line


    Performed under reducing conditions.

    Predicted band size : 134 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with ab81440 at 1µg/ml and goat anti-mouse-HRP.
  • Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.

References for Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)

This product has been referenced in:
  • Harrison H  et al. Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling. Breast Cancer Res 15:R21 (2013). WB ; Human . Read more (PubMed: 23497505) »

See 1 Publication for this product

Product Wall

I am very pleased to hear you would like to accept our offer and test ab81440 in IHC on human tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for ICH on human tissue an...

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Thank you for contacting me with this additional information.

I would be happy to create 100% abreview discount codes for each of these applications for you. However each code has to be associated with a single vial. Therefore if you want fo...

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Thank you for contacting Abcam.

I would be glad to create a 100% Abreview discount code for you. Would you be testing ab81440 in human tissues? Further for clarity, do you wish to perform IHC using fluorescence on frozen tissues?

...

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Thank you very much for your telephone call yesterday afternoon.

As discussed on the phone, to our knowledge, ab81440has not been tested in ICC-IF or IHC-Fr and regrettably there are no suitably tested alternative mouse monoclonalsavailablet...

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