Recombinant Anti-EHD1 antibody [EPR4954] (ab109311)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4954] to EHD1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-EHD1 antibody [EPR4954]
See all EHD1 primary antibodies -
Description
Rabbit monoclonal [EPR4954] to EHD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, Human testis, SW480, SK-OV-3, Human brain, HeLa, Mouse brain, and Rat brain lysates. IHC-P: human thyroid carcinoma, kidney and tonsil, mouse lung, and rat lung tissues. IP: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4954 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
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IP |
1/10 - 1/100.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
(heat to 98°C, allow to cool for 10-20 minutes) 1:120 in human tissues / 1:1600 in mouse and rat tissues |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa). |
IP
1/10 - 1/100. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (heat to 98°C, allow to cool for 10-20 minutes) 1:120 in human tissues / 1:1600 in mouse and rat tissues |
Target
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Function
Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. -
Tissue specificity
Highly expressed in testis. -
Sequence similarities
Contains 1 EF-hand domain.
Contains 1 EH domain. -
Domain
The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins. -
Cellular localization
Cell membrane. Early endosome membrane. Recycling endosome membrane. Associated with intracellular tubulovesicular structures. - Information by UniProt
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Database links
- Entrez Gene: 10938 Human
- Entrez Gene: 13660 Mouse
- Entrez Gene: 293692 Rat
- Omim: 605888 Human
- SwissProt: Q9H4M9 Human
- SwissProt: Q9WVK4 Mouse
- SwissProt: Q641Z6 Rat
- Unigene: 523774 Human
see all -
Alternative names
- CDABP0131 antibody
- EH domain containing 1 antibody
- EH domain-containing protein 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling EHD1 with purified ab109311 at 1/120 dilution (2.85 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab109311 (purified ) at 1/30 dilution (2ug) immunoprecipitating EHD1 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab109311 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109311 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/1000 dilution ((unpurified))
Lane 1 : HepG2 cell lysates
Lane 2 : Human testis lysates
Lane 3 : SW480 cell lysates
Lane 4 : SK-OV-3 cell lysates
Lane 5 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemical analysis of EHD1 in paraffin embedded Human kidney tissue, using unpurified ab109311 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of EHD1 in paraffin embedded Human tonsil tissue, using unpurified ab109311 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab109311 has been referenced in 19 publications.
- Jones T et al. Differential requirements for the Eps15 homology domain proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic 23:360-373 (2022). PubMed: 35510564
- Jones T et al. Eps15 Homology Domain Protein 4 (EHD4) is required for Eps15 Homology Domain Protein 1 (EHD1)-mediated endosomal recruitment and fission. PLoS One 15:e0239657 (2020). PubMed: 32966336
- Dhawan K et al. Sorting nexin 17 (SNX17) links endosomal sorting to Eps15 homology domain protein 1 (EHD1)-mediated fission machinery. J Biol Chem 295:3837-3850 (2020). PubMed: 32041776
- Tom EC et al. EHD1 and RUSC2 Control Basal Epidermal Growth Factor Receptor Cell Surface Expression and Recycling. Mol Cell Biol 40:N/A (2020). PubMed: 31932478
- Zhou Q et al. EHD1 impairs decidualization by regulating the Wnt4/ß-catenin signaling pathway in recurrent implantation failure. EBioMedicine 50:343-354 (2019). PubMed: 31707150