![Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299) image](#EHHADH-Primary-antibodies-ab110299-3.gif)
![Immunocytochemistry/ Immunofluorescence - Anti-EHHADH antibody [7F6AF11] (ab110299) image](#EHHADH-Primary-antibodies-ab110299-4.gif)
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Product name
Anti-EHHADH antibody [7F6AF11]
See all EHHADH products (5) ...
Description
Mouse monoclonal [7F6AF11] to EHHADH
Tested applications
IP, ICCmore details
Cross reactivity
Reacts with
Human
Immunogen
Human liver mitochondria.
Positive control
Human liver mitochondria and HpG2 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
7F6AF11
Isotype
IgG3
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Fatty acids
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ICC: Use a concentration of 1 µg/ml.
Target
Tissue specificity
Liver and kidney. Lower amounts seen in the brain.
Pathway
Lipid metabolism; fatty acid beta-oxidation.
Sequence similarities
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
Post-translational
modifications
Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionQ08426
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 3 hydroxyacyl CoA dehydrogenase antibody
- 3,2 trans enoyl CoA isomerase antibody
- 3-hydroxyacyl-CoA dehydrogenase antibody
see all
Database links
- Entrez Gene: 1962 Human
- Omim: 607037 Human
- SwissProt: Q08426 Human
- Unigene: 429879 Human
Anti-EHHADH antibody [7F6AF11] images:
Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)
![Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)](/ps/datasheet/images/110/ab110299/EHHADH-Primary-antibodies-ab110299-3.gif)
EHHADH immunocaptured from Human liver mitochondria using ab110299.
Immunocytochemistry/ Immunofluorescence - Anti-EHHADH antibody [7F6AF11] (ab110299)
![Immunocytochemistry/ Immunofluorescence - Anti-EHHADH antibody [7F6AF11] (ab110299)](/ps/datasheet/images/110/ab110299/EHHADH-Primary-antibodies-ab110299-4.gif)
Immunocytochemistry image of EHHADH stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated ab110299 at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.
References for Anti-EHHADH antibody [7F6AF11] (ab110299)
ab110299 has not yet been referenced specifically in any publications.
Publishing research using ab110299? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"