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Anti-EHHADH antibody (ab72795) at 1/500 dilution + Human liver tissue lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 79 kDa
Observed band size : 79 kDa
All lanes : Anti-EHHADH antibody (ab72795) at 1/500 dilution
Lane 1 : EHHADH transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 79 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Immunofluorescence analysis of fixed and permeabilised HeLa cells using purified ab72795 at 10µg/ml followed by a fluorescein-conjugated secondary antibody.
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