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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab72795 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - EHHADH antibody (ab72795)
Anti-EHHADH antibody (ab72795) at 1/500 dilution + Human liver tissue lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 79 kDa
Observed band size : 79 kDa
Western blot - EHHADH antibody (ab72795)
All lanes : Anti-EHHADH antibody (ab72795) at 1/500 dilution
Lane 1 : EHHADH transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 79 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - EHHADH antibody (ab72795)
Immunofluorescence analysis of fixed and permeabilised HeLa cells using purified ab72795 at 10µg/ml followed by a fluorescein-conjugated secondary antibody.
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