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Products:Signal Transduction >> Metabolism >> Lipid metabolism
Overview
Product name
Anti-EHHADH antibody
See all EHHADH products (5) ...
Description
Mouse polyclonal to EHHADH
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein (Human) (NP_001957.2)
Positive control
Human liver tissue lysate HeLa cells EHHADH transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 10 µg/ml. Antibody must be purified (with Protein A) before use.
WB: 1/500 - 1/1000. Predicted molecular weight: 79 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Tissue specificity
Liver and kidney. Lower amounts seen in the brain.
Pathway
Lipid metabolism; fatty acid beta-oxidation.
Sequence similarities
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
Post-translational
modifications
Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionQ08426
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 3 hydroxyacyl CoA dehydrogenase antibody
- 3,2 trans enoyl CoA isomerase antibody
- 3-hydroxyacyl-CoA dehydrogenase antibody
see all
Database links
- Entrez Gene: 1962 Human
- Omim: 607037 Human
- SwissProt: Q08426 Human
- Unigene: 429879 Human
Anti-EHHADH antibody images:
Western blot - EHHADH antibody (ab72795)
Anti-EHHADH antibody (ab72795) at 1/500 dilution + Human liver tissue lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 79 kDa
Observed band size : 79 kDa
Western blot - EHHADH antibody (ab72795)
All lanes : Anti-EHHADH antibody (ab72795) at 1/500 dilution
Lane 1 : EHHADH transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 79 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - EHHADH antibody (ab72795)
Immunofluorescence analysis of fixed and permeabilised HeLa cells using purified ab72795 at 10µg/ml followed by a fluorescein-conjugated secondary antibody.
References for Anti-EHHADH antibody (ab72795)
ab72795 has not yet been referenced specifically in any publications.
Publishing research using ab72795? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"