Anti-EHHADH antibody (ab94948)

Overview

• Product nameAnti-EHHADH antibodySee all EHHADH primary antibodies ...
• Description
  Rabbit polyclonal to EHHADH
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Recombinant fragment, corresponding to amino acids 535-723 of Human EHHADH (NM_001966).

• Positive controlHepG2 and 293T cell lysates; Human fetal kidney tissue

Properties

• FormLyophilised:Reconstitute in 200ul sterile H2O.
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

Applications

Target

• Tissue specificityLiver and kidney. Lower amounts seen in the brain.
• PathwayLipid metabolism; fatty acid beta-oxidation.
• Sequence similaritiesIn the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
  In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
• Post-translational
  modificationsAcetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids.
• Cellular localizationPeroxisome.

Target information above from: UniProt accession Q08426 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1962 Human
  • Entrez Gene: 74147 Mouse
  • Entrez Gene: 171142 Rat
  • Omim: 607037 Human
  • SwissProt: Q08426 Human
  • SwissProt: Q9DBM2 Mouse
  • SwissProt: P07896 Rat
  • Unigene: 429879 Human

  • Unigene: 28100 Mouse
  • Unigene: 3671 Rat

  see all

• Alternative names
  3 hydroxyacyl CoA dehydrogenase antibody3,2 trans enoyl CoA isomerase antibody3-hydroxyacyl-CoA dehydrogenase antibody

  ECHD antibodyECHP_HUMAN antibodyEHHADH antibodyEnoyl Coenzyme A, hydratase/3 hydroxyacyl Coenzyme A dehydrogenase antibodyL 3 hydroxyacyl CoA dehydrogenase antibodyL bifunctional protein, peroxisomal antibodyL PBE antibodyLBFP antibodyLBP antibodyMGC120586 antibodyMS730 antibodyPBE antibodyPBFE antibodyPeroxisomal bifunctional enzyme antibodyPeroxisomal enoyl CoA hydratase antibody

  see all

Anti-EHHADH antibody images

• 

  Western blot - EHHADH antibody (ab94948)
  All lanes : Anti-EHHADH antibody (ab94948) at 1/500 dilution
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : 293T cell lysate
  
  
  Predicted band size : 79 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EHHADH antibody (ab94948)
  ab94948, at 1/100 dilution, staining EHHADH in formalin-fixed, paraffin-embedded Human fetal kidney tissue by Immunohistochemistry.