![Western blot - EID1 antibody [26] (ab78323) image](#EID1-Primary-antibodies-ab78323-7.JPG)
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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-EID1 antibody
See all EID1 products (2) ...
Description
Mouse monoclonal to EID1
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Cynomolgus Monkey, Orangutan
Immunogen
Synthetic peptide: YEKTPFDQLAFIEELFSLMVVNRLTEELGC, corresponding to amino acids 159 - 187 of Human EID1
YEKTPFDQLA FIEELFSLMV VNRLTEELGC
Positive control
extracts from MCF7 cells transfected with EID1
General notes
Clone number: #26
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
ab78323 was purified by propriety chromatography under mild conditions as IgG fraction from serum free growth medium of mouse hybridoma clone and sterilized by filtration.
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Rb family
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HAT
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cAMP
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Western blot - EID1 antibody [26] (ab78323)
![Western blot - EID1 antibody [26] (ab78323) image](/ps/datasheet/images/78/ab78323/EID1-Primary-antibodies-ab78323-7.JPG)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 21 kDa.
Target
Function
Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.
Tissue specificity
Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.
Developmental stage
Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level).
Post-translational
modifications
Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit.
Cellular localization
Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm.
Target information above from: UniProt accessionQ9Y6B2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 21 kDa pRb associated protein antibody
- 21 kDa pRb-associated protein antibody
- C15orf3 antibody
see all
Database links
- Entrez Gene: 506056 Cow
- Entrez Gene: 23741 Human
- Entrez Gene: 58521 Mouse
- Entrez Gene: 100171915 Orangutan
- Entrez Gene: 499882 Rat
- Omim: 605894 Human
- SwissProt: Q9Y6B2 Human
- SwissProt: Q9DCR4 Mouse
see all
Anti-EID1 antibody images:
Western blot - EID1 antibody [26] (ab78323)
All lanes : Anti-EID1 antibody (ab78323) at 1 µg/ml
Lane 1 : extracts from MCF7 cells transfected with control
vector pCMV1
Lane 2 : extracts from MCF7 cells transfected with the EID1 expression
vector pcDNA3/EID1
Secondary
HRP-conjugated mouse IgG
Predicted band size : 21 kDa
Observed band size : 21 kDa
References for Anti-EID1 antibody (ab78323)
ab78323 has not yet been referenced specifically in any publications.
Publishing research using ab78323? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"