Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Could you please tell me ab32157 is directed against which amino acids (numbers) of eIF2alpha? |
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ANSWER: |
Thank you for your enquiry. ab32157 is a phospho specific antibody specific to the phosphorylation site at AA51. So obviously, the peptide spans that region. Most peptides are around 15 AA long, so you could safely predict it covers around AA42-58. I hope this information will be useful to you. Should you require any other information, please do not hesitate to contact me. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution + PC12 cell lysate
Predicted band size : 36 kDa
Observed band size : 36 kDa
Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.
Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1:500 dilution.
ab32157 at 1/100 staining human epithelial cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 1 hour. An Alexa-Fluor ® 647 conjugated goat anti-rabbit IgG was used as the secondary.
This image is courtesy of an Abreview submitted by Mr mike lindquist
ab32157 showing positive staining in Breast carcinoma tissue.
ab32157 showing positive staining in Cervical carcinoma tissue.
ab32157 showing positive staining in Colonic adenocarcinoma tissue.
ab32157 showing positive staining in Hepatocellular carcinoma tissue.
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