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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Regulation
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Read our guarantee »Anti-EIF3S2 antibody
See all EIF3S2 products (4) ...
Chicken polyclonal to EIF3S2
WB, ELISAmore details
Reacts with
Mouse, Human
Synthetic peptide, corresponding to N terminal amino acids 1-230 of Human EIF3S2.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: PBS
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Immunogen affinity purified
Polyclonal
IgY
Our Abpromise guarantee covers the use of ab37526 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ELISA: Use a concentration of 1 µg/ml.
EIF3S2 is the largest of the EIFs. It consists of at least 10 nonidentical subunits in mammals. In S. cerevisiae the p39 subunit contains WD repeats; these are thought to mediate protein-protein interactions. The p39 protein appears to be essential for maintaining the integrity of the yeast EIF3 complex. The mammalian EIF3-p36 subunit is homologous to yeast p39.
Cytoplasmic
ELISA - EIF3S2 antibody (ab37526)

Direct ELISA test. Serial dilutions of Human EIF3S2 protein were used as test antigen. ab37526 was used as test antibody at 1µg/ml. Goat anti-IgY-HRP was used as secondary antibody.
Western blot - EIF3S2 antibody (ab37526)

Anti-EIF3S2 antibody (ab37526) at 1/2000 dilution + E. coli derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP at 1/1000 dilution
Predicted band size : 37 kDa
Observed band size : 37 kDa
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