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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-EKLF / KLF1 antibody - ChIP Grade
See all EKLF / KLF1 products (7) ...
Description
Goat polyclonal to EKLF / KLF1 - ChIP Grade
Tested applications
ChIP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: ATAETALPSISTLT, corresponding to N terminal amino acids 2-15 of Human KLF1.
ATAETALPSI STLT
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Krueppel like factor
Epigenetics and Nuclear Signaling >> Transcription >> Krueppel like factor
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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ChIP - EKLF / KLF1 antibody - ChIP Grade (ab2483)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab2483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (PubMed: 19074263)
WB: Use at an assay dependent dilution. (Preliminary experiments gave an approx 52kDa band in K562 cell lysate after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of 38kDa according to NP_006554. The 52kDa band was successfully blocked by incubation with the immunizing peptide)
Target
Function
Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
Tissue specificity
Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
Involvement in disease
Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
Post-translational
modifications
Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.
Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.
Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.
Cellular localization
Nucleus. Colocalizes with SUMO1 in nuclear speckles.
Target information above from: UniProt accessionQ13351
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- EKLF antibody
- EKLF PEN antibody
- Erythroid krueppel like transcription factor (EKLF) (Erythroid transcription factor) antibody
see all
Database links
- Entrez Gene: 10661 Human
- Entrez Gene: 16596 Mouse
- Omim: 600599 Human
- SwissProt: Q13351 Human
- SwissProt: Q6PIJ5 Human
- SwissProt: P46099 Mouse
- Unigene: 37860 Human
- Unigene: 4847 Mouse
Anti-EKLF / KLF1 antibody - ChIP Grade images:
ChIP - EKLF / KLF1 antibody - ChIP Grade (ab2483)
Detection of EKLF / KLF1 binding at the region upstream of the transcription start site of the HBE1 gene by ChIP with ab2483. Chromatin from CD43+ cells was cross-linked prior to ChIP using ab2483 at a concentration of 0.2 µg/µg chromatin. DNA was quantified using RT-PCR. Please see abreview by Genpathway Inc for additional details.
This image is courtesy of an Abreview submitted by Genpathway Inc
References for Anti-EKLF / KLF1 antibody - ChIP Grade (ab2483)
This product has been referenced in:
- Alhashem Yet al. Transcription factors KLF1 and KLF2 positively regulate embryonic and fetal {beta}-globin genes through direct promoter binding. J Biol Chem : (2011). ChIP; Mouse.Read more (PubMed: 21610079) »
- Singleton BKet al. Mutations in the second zinc finger of human EKLF reduce promoter affinity but give rise to benign and disease phenotypes. Blood 118:3137-45 (2011).Read more (PubMed: 21778342) »
See all 3 publications for this product
Publishing research using ab2483? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"