Anti-ELK1 antibody (ab125085)
Key features and details
- Rabbit polyclonal to ELK1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ELK1 antibody
See all ELK1 primary antibodies -
Description
Rabbit polyclonal to ELK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish -
Immunogen
Synthetic peptide, corresponding to between amino acids 378-428 of Human ELK1 (NP_005220.2).
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Positive control
- 293T and HeLa whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab125085 is affinity purified using an epitope specific to ELK1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 45 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. -
Tissue specificity
Lung and testis. -
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
Post-translational
modificationsSumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2002 Human
- Entrez Gene: 13712 Mouse
- Entrez Gene: 314436 Rat
- Omim: 311040 Human
- SwissProt: P19419 Human
- SwissProt: P41969 Mouse
- SwissProt: A4GTP4 Rat
- Unigene: 181128 Human
see all -
Alternative names
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
see all
Images
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All lanes : Anti-ELK1 antibody (ab125085) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 3 minutes -
Detection of ELK1 by Western Blot of Immunprecipitate.
ab125085 at 1 µg/ml staining ELK1 in 293T whole cell lysate immunoprecipitated using ab125085 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab125085 has been referenced in 1 publication.
- Li K et al. BCL6 is regulated by the MAPK/ELK1 axis and promotes KRAS-driven lung cancer. J Clin Invest 132:N/A (2022). PubMed: 36377663