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Product name
Anti-ELK1 antibody
See all ELK1 products (16) ...
Description
Rabbit polyclonal to ELK1
Specificity
ab31484 detects endogenous levels of Elk-1 protein around Threonine 417.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human Elk-1 around the phosphorylation site of Threonine 417.
Positive control
breast carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 45 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
Target
Function
Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Tissue specificity
Lung and testis.
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Post-translational
modifications
Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP19419
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
see all
Database links
- Entrez Gene: 2002 Human
- Entrez Gene: 13712 Mouse
- Entrez Gene: 314436 Rat
- Omim: 311040 Human
- SwissProt: P19419 Human
- SwissProt: P41969 Mouse
- Unigene: 181128 Human
- Unigene: 405823 Mouse
see all
Anti-ELK1 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - ELK1 antibody (ab31484)
ab31484 at 1/50, Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using ab31484, Right: The same antibody preincubated with synthesized peptide.
Western blot - ELK1 antibody (ab31484)
All lanes : Anti-ELK1 antibody (ab31484) at 1 µg/ml
Lane 1 :
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 45 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.
References for Anti-ELK1 antibody (ab31484)
ab31484 has not yet been referenced specifically in any publications.
Publishing research using ab31484? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"