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Product name
Anti-ELK1 (phospho T417) antibody
See all ELK1 products (16) ...
Description
Rabbit polyclonal to ELK1 (phospho T417)
Specificity
ab28817 recognises Phospho-ELK-1(Thr417).
Tested applications
ELISA, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phospho-peptide derived from human ELK-1 around the phosphorylation site of Threonine 417.
Positive control
Breast carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
-
Immunohistochemistry (Paraffin-embedded sections) - ELK1 (phospho T417) antibody (ab28817)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000.ELISA: 1/10000.
IP: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100.
Target
Function
Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
Tissue specificity
Lung and testis.
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Post-translational
modifications
Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP19419
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
see all
Database links
- Entrez Gene: 2002 Human
- Entrez Gene: 13712 Mouse
- Omim: 311040 Human
- SwissProt: P19419 Human
- SwissProt: P41969 Mouse
- Unigene: 181128 Human
- Unigene: 405823 Mouse
Anti-ELK1 (phospho T417) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - ELK1 (phospho T417) antibody (ab28817)
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-ELK-1(Thr417) Antibody (ab28817); Right: The same antibody preincubated with synthesized phosphopeptide.
References for Anti-ELK1 (phospho T417) antibody (ab28817)
ab28817 has not yet been referenced specifically in any publications.
Publishing research using ab28817? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"