Anti-ELL3 antibody (ab67415)
Key features and details
- Mouse polyclonal to ELL3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ELL3 antibody -
Description
Mouse polyclonal to ELL3 -
Host species
Mouse -
Specificity
Reactive against mammalian transfected lysate. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human ELL3 aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Transfected 293T lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). |
Target
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Relevance
ELL3 is a testis-specific RNA polymerase II elongation factor which increases the catalytic rate of transcription elongation. ELL3 is approximately 50% similar to both ELL and ELL2. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 80237 Human
- Omim: 609885 Human
- SwissProt: Q9HB65 Human
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Alternative names
- Elongation Factor RNA Polymerase II-Like 3 antibody
- RNA polymerase II elongation factor ELL3 antibody
Images
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All lanes : Anti-ELL3 antibody (ab67415) at 1/500 dilution
Lane 1 : ELL3 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 45 kDa
Observed band size: 43.78 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (3)
ab67415 has been referenced in 3 publications.
- Chen Y et al. The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells. Front Cell Neurosci 15:735723 (2021). PubMed: 34658793
- Lee JY et al. Ell3 functions as a critical decision maker at the crossroad between stem cell senescence and apoptosis. Stem Cell Res Ther 10:32 (2019). PubMed: 30654843
- Ahn HJ et al. Ell3 stabilizes p53 following CDDP treatment via its effects on ubiquitin-dependent and -independent proteasomal degradation pathways in breast cancer cells. Oncotarget 6:44523-37 (2015). WB . PubMed: 26540344