Recombinant Anti-ELMO1 antibody [EPR12919] - BSA and Azide free (ab222227)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12919] to ELMO1 - BSA and Azide free
- Suitable for: IP, ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ELMO1 antibody [EPR12919] - BSA and Azide free
See all ELMO1 primary antibodies -
Description
Rabbit monoclonal [EPR12919] to ELMO1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, WB, Flow Cyt (Intra)more details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human placenta and Jurkat lysates. ICC/IF: K562 cells
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General notes
ab222227 is the carrier-free version of ab174298.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12919 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 84 kDa. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. -
Tissue specificity
Widely expressed, with a higher expression in the spleen and placenta. -
Sequence similarities
Contains 1 ELMO domain.
Contains 1 PH domain. -
Post-translational
modificationsPhosphorylated by HCK. -
Cellular localization
Cytoplasm. Cell membrane. Translocation to plasma membrane seems to be mediated by DOCK1 and CRK. - Information by UniProt
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Database links
- Entrez Gene: 9844 Human
- Entrez Gene: 140580 Mouse
- Entrez Gene: 361251 Rat
- Omim: 606420 Human
- SwissProt: Q92556 Human
- SwissProt: Q8BPU7 Mouse
- Unigene: 656638 Human
- Unigene: 342392 Mouse
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Alternative names
- CED 12 antibody
- Ced 12 homolog 1 antibody
- Ced 12 homolog antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of K562 labeling ELMO1 with ab222227 at 1/250 dilution. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI was used to counter stain nuclei (blue).
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LR73 cells were transfected with the indicated plasmids and stained with Alexa Fluor 488–conjugated phalloidin and anti-Elmo1 antibody.
One day after transfection, cells were washed with cold PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100 for 5 min. Next, permeabilized cells were blocked with 1% BSA for 30 min and stained with Alex Fluor 594–conjugated phalloidin and anti-Elmo1 antibody for 1 h at room temperature.
(After Figure 5 of Kim et al).
http://creativecommons.org/licenses/by/4.0/.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab174298).
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This Flow Cyt data was generated using the same atni-ELMO1 antibody clone,EPR12919, in a different buffer formulation (cat
ab174298).
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling ELMO1 with unpurified ab174298 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab222227 has not yet been referenced specifically in any publications.