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Overview

  • Product nameELMO2 protein (Tagged-His Tag)See all ELMO2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 491-720 (230 aa) of Human ELMO2 with a N terminal His tag; predicted MWt 32kDa (NP_573403.1).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 141 µl aqua dedistilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesSLDQFKSKLRSLSYSEILRLRQSERMSQDDFQSPPIVELR EKIQPEILELIKQQ RLNRLCEGSSFRKIGNRRRQERFW YCRLALNHKVLHYGDLDDNPQGEVTFESLQ EKIPVADI KAIVTGKDCPHMKEKSALKQNKEVLELAFSILYDPDETLN FIAPNK YEYCIWIDGLSALLGKDMSSELTKSDLDTLLS MEMKLRLLDLENIQIPEAPPPI PKEPSSYDFVYHYG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91833 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        CED 12Ced 12 homolog 2CED 12 homolog A
        CED 12ACED-12ced-12 homolog 2CED12CED12 homolog ACED12AELMO 2ELMO-2ELMO2ELMO2_HUMANEngulfment and cell motility 2Engulfment and cell motility 2 (ced 12 homolog, C. elegans)Engulfment and cell motility protein 2FLJ11656hCED 12AhCED-12AhCED12AKIAA1834PH domain protein CED12AProtein ced 12 homolog AProtein ced-12 homolog A
      see all
  • FunctionInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
  • Tissue specificityWidely expressed, with a higher expression in skeletal muscle, kidney and placenta.
  • Sequence similaritiesContains 1 ELMO domain.
    Contains 1 PH domain.
  • Cellular localizationCytoplasm. Cytoplasm > cytosol. Membrane.

    • Target information above from: UniProt accession Q96JJ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ELMO2 protein (Tagged-His Tag) images

    • SDS-PAGE - ELMO2 protein (Tagged-His Tag) (ab91833)
      SDS-PAGE - ELMO2 protein (Tagged-His Tag) (ab91833)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for ELMO2 protein (Tagged-His Tag) (ab91833)

    ab91833 has not yet been referenced specifically in any publications.

    Publishing research using ab91833 ? Please let us know so that we can cite the reference in this datasheet

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