![Western blot - EMSY antibody [Y474] (ab32329) image](#WB EMSY.jpg)
![Immunocytochemistry/ Immunofluorescence - EMSY antibody [Y474] (ab32329) image](#IF EMSY.jpg)
 image](#EMSY-Primary-antibodies-ab32329-1.jpg)
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Related products
- Secondary antibodies
- Lysates
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Read our guarantee »Overview
Product name
Anti-EMSY antibody [Y474]
See all EMSY products (5) ...
Description
Rabbit monoclonal [Y474] to EMSY
Specificity
ab32329 recognises EMSY.
Tested applications
WB, ICC/IF, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Epitope
ab32329 reacts with an epitope located in the C terminal region if EMSY.
Positive control
MCF7 cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
Y474
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 141 kDa.
ICC/IF: 1/250 - 1/500.
Flow Cyt: 1/150.
IP
IP: 1/50.IP: 1/50.
Application notes
Is unsuitable for or IHC.
Target
Relevance
EMSY binds BRCA2 within a region (exon 3) deleted in cancer. EMSY is capable of silencing the activation potential of BRCA2 exon 3, associates with chromatin regulators HP1 and BS69, and localizes to sites of repair following DNA damage. Its levels are amplified in breast and ovarian cancer.
Cellular localization
Nuclear. Localizes to DNA damage markers in irradiated cells; suggesting that it participates in the DNA repair process.
Alternative names
- C11orf30 antibody
- Chromosome 11 open reading frame 30 antibody
- EMSY protein antibody
see all
Database links
- Entrez Gene: 56946 Human
- Omim: 608574 Human
- SwissProt: Q7Z589 Human
- SwissProt: Q8BMB0 Mouse
- Unigene: 352588 Human
Anti-EMSY antibody [Y474] images:
Western blot - EMSY antibody [Y474] (ab32329)
![Western blot - EMSY antibody [Y474] (ab32329)](/ps/datasheet/Images/32/ab32329/WB EMSY.jpg)
Anti-EMSY antibody [Y474] (ab32329) at 1/1000 dilution + MCF7 cell lysate
Predicted band size : 141 kDa
Observed band size : 150 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - EMSY antibody [Y474] (ab32329)
![Immunocytochemistry/ Immunofluorescence - EMSY antibody [Y474] (ab32329)](/ps/datasheet/Images/32/ab32329/IF EMSY.jpg)
Immunofluorescent analysis of EMSY expression in MCF7 cells using 1/250 ab32329.
Flow Cytometry-Anti-EMSY antibody [Y474](ab32329)
](/ps/datasheet/images/32/ab32329/EMSY-Primary-antibodies-ab32329-1.jpg)
Overlay histogram showing MCF7 cells stained with ab32329 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32329, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-EMSY antibody [Y474] (ab32329)
ab32329 has not yet been referenced specifically in any publications.
Publishing research using ab32329? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"