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Recombinant fragment corresponding to EMSY ENT domain:
MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGDLTKEKKDLLG ELSKVLSISTER HRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRL, corresponding to amino acids 1-100 of Human EMSY - FPLC purified.
Our Abpromise guarantee covers the use of ab4579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/500. Predicted molecular weight: 141 kDa.|
|ICC||Use at an assay dependent dilution.|
Western blot of EMSY on MCF-7 cell lysate.
Lane 1: ab4579 at 1ug/ml with 2% BSA blocking.
Lane 2: ab4579 at 1ug/ml with 3% milk blocking.
Lane 3: ab123 at 1ug/ml with 2% BSA blocking.
Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.
Lane 1 & 2 - Exposure time: 2 min.
Lane 3 - Exposure time: 2 sec.