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Anti-EMSY antibody (ab4579)

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Overview

Product name

Anti-EMSY antibody
See all EMSY products (5) ...

Description

Goat polyclonal to EMSY

Specificity

This antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY.

Tested applications

IF, WB, ICCmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment corresponding to EMSY ENT domain: MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGD LTKEKKDLLGELSKVLSISTER HRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRL, corresponding to amino acids 1-100 of Human EMSY - FPLC purified.

Positive control

MCF-7 cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> Methyl Lysine

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
WB: 1/500. Predicted molecular weight: 141 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD. However, the independently raised ab123 has been used to confirm the specificity of this antibody.

Target

Relevance

EMSY binds BRCA2 within a region (exon 3) deleted in cancer. EMSY is capable of silencing the activation potential of BRCA2 exon 3, associates with chromatin regulators HP1 and BS69, and localizes to sites of repair following DNA damage. Its levels are amplified in breast and ovarian cancer.

Cellular localization

Nuclear. Localizes to DNA damage markers in irradiated cells; suggesting that it participates in the DNA repair process.

Alternative names

  • C11orf30 antibody
  • Chromosome 11 open reading frame 30 antibody
  • EMSY protein antibody
  • FLJ90741 antibody
  • GL002 antibody
  • Protein EMSY antibody
see all

Database links

Anti-EMSY antibody images:

  Western blot

Western blot

Lane 1 : Anti-EMSY antibody (ab4579) at 1/500 dilution
Lane 2 : Anti-EMSY antibody (ab123) at 1/500 dilution

Lane 1 : MCF-7 cell lysate
Lane 2 : MCF-7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6741) at 1/5000 dilution
Lane 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/5000 dilution


Performed under reducing conditions.

Lane 1 - Exposure time : 3 min.
Lane 2 - Exposure time : 10 sec.

Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD (even though the predicted size is 141kD). However, that the same size band is detected by both ab123 and ab4579 (raised with immunogens of different sequence) confirms the specificity of these antibodies.

References for Anti-EMSY antibody (ab4579)

ab4579 has not yet been referenced specifically in any publications.

Publishing research using ab4579? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"