You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-EMSY antibodySee all EMSY primary antibodies ...
  • Description
    Goat polyclonal to EMSY
  • SpecificityThis antibody detects a band of the same size by Western blot as ab123, which was raised with a recombinant fragment of EMSY with no sequence in common with the immunogen for this antibody (and was raised 7 years previous to this antibody). This confirms that the band detected is EMSY.
  • Tested applicationsICC/IF, WB, ICC more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to EMSY ENT domain: MPVVWPTLLDLSRDECKRILRKLELEAYAGVISALRAQGD LTKEKKDLLGELSKVLSISTER HRAEVRRAVNDERLTTIAHNMSGPNSSSEWSIEGRRL, corresponding to amino acids 1-100 of Human EMSY - FPLC purified.

  • Positive controlMCF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab4579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
WB
ICC
  • Application notesICC: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.
    WB: 1/500. Predicted molecular weight: 141 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Variability in the size at which EMSY runs by Western blot has been experienced with different protein marker systems. EMSY has been observed to run at between 115 and 150kD. However, the independently raised ab123 has been used to confirm the specificity of this antibody.

    • Target

    • RelevanceEMSY binds BRCA2 within a region (exon 3) deleted in cancer. EMSY is capable of silencing the activation potential of BRCA2 exon 3, associates with chromatin regulators HP1 and BS69, and localizes to sites of repair following DNA damage. Its levels are amplified in breast and ovarian cancer.
    • Cellular localizationNuclear. Localizes to DNA damage markers in irradiated cells; suggesting that it participates in the DNA repair process.
    • Database links
      • Alternative names
          C11orf30 antibodyChromosome 11 open reading frame 30 antibodyEMSY protein antibody
          FLJ90741 antibodyGL002 antibodyProtein EMSY antibody
        see all

      References for Anti-EMSY antibody (ab4579)

      ab4579 has not yet been referenced specifically in any publications.

      Publishing research using ab4579 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab4579 Submit a Question for ab4579

      There are currently no Abreviews or Questions for ab4579.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"