Anti-EMX2 antibody (ab86004)

Overview

• Product nameAnti-EMX2 antibodySee all EMX2 primary antibodies ...
• Description
  Rabbit polyclonal to EMX2
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Recombinant Fragment
  Predicted to work with: Mouse, Human
• Immunogen

  Three synthetic peptides derived from human EMX2, conjugated to KLH.

• Positive controlAn anti thio tested on an induced culture of E.coli.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
  Constituents: Whole serum
• PurityWhole antiserum
• Purification notesCrude serum, final bleed
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

  • Neuroscience
  • Cell Type Marker
  • Neural Stem Cell marker

Applications

Target

• FunctionTranscription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
• Tissue specificityCerebral cortex.
• Involvement in diseaseDefects in EMX2 are the cause of schizencephaly (SCHZC) [MIM:269160]. Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro-spinal fluid.
• Sequence similaritiesBelongs to the EMX homeobox family.
  Contains 1 homeobox DNA-binding domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q04743 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2018 Human
  • Entrez Gene: 13797 Mouse
  • Omim: 600035 Human
  • SwissProt: Q04743 Human
  • SwissProt: Q04744 Mouse
  • Unigene: 202095 Human
  • Unigene: 245394 Mouse

• Alternative names
  Empty spiracles homeobox 2 antibodyEmpty spiracles homolog 2 (Drosophila) antibodyEmpty spiracles homolog 2 antibody

  Empty spiracles like protein 2 antibodyEmpty spiracles-like protein 2 antibodyEMX 2 antibodyEMX2 antibodyEMX2_HUMAN antibodyHomeobox protein EMX 2 antibodyHomeobox protein EMX2 antibody

  see all

Anti-EMX2 antibody images

• 

  Western blot - EMX2 antibody (ab86004)
  Lane 1 : Anti-EMX2 antibody (ab86004) at 1/1000 dilution (in 5% non fat milk PBST solution incubated for 60 minutes)
  Lane 2 : Anti-EMX2 antibody (ab86004) (in 5% non fat milk PBST solution incubated for 60 minutes)
  
  Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution)
  Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution)
  
  Secondary
  anti-Rabbit IgG HRP conjugate incubated for 60 minutes at 1/1000 dilution
  
  Predicted band size : 28 kDa
  Observed band size : 42 kDa (why is the actual band size different from the predicted?)