Overview

  • Product nameENO1 Human Activity Assay Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Cell extract 9 9%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    125 ng/ml
  • Range
    125 ng/ml - 2000 ng/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 63 % - %
    Serum 140 % - %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Notes

    ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.

  • Tested applicationsFunctional Studiesmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • MS988

Applications

Our Abpromise guarantee covers the use of ab117994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

ENO1 Human Activity Assay Kit images

  • Analysis of recombinant ENO1 (ab89248) and the neuron-specific recombinant ENO2 (ab78797), each at 2000 ng/mL. The relative ENO activity was determined from HepG2 standard curve as described in the protocol. The data show that the activity of the neuron-specific ENO2 can’t be detected.
  • Example of dilution series prepared from HepG2 cells extract.
  • Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994.
  • Scheme of reactions used in this kit. Abbreviations are as follows: enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).

Protocols

References for ENO1 Human Activity Assay Kit (ab117994)

This product has been referenced in:
  • Bottermann K  et al. Systematic Analysis Reveals Elongation Factor 2 and a-Enolase as Novel Interaction Partners of AKT2. PLoS One 8:e66045 (2013). Read more (PubMed: 23823123) »

See 1 Publication for this product

Product Wall

Regarding LDH control:
Please note that the LDH is simply added to the rehydrated 'Reagent Mix', along with PK, to produce the 1x Acitvity Solution.

Regarding data analysis:
If data collected in a kinetic mode, they are presented as...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"