ENO1 Human Activity Assay Kit (ab117994)
- Product nameENO1 Human Activity Assay Kit
Intra-assay Sample n Mean SD CV% Cell extract 9 9% Inter-assay Sample n Mean SD CV% Cell extract 4 11%
- Tests1 x 96 well plate
- Sample typeCell culture extracts, Tissue Extracts
- Assay typeEnzyme activity
- Sensitivity125 ng/ml
- Range125 ng/ml - 2000 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 63 % - % Serum 140 % - %
- Species reactivityReacts with: Human
Does not react withMouse, Rat
ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Blocking Buffer 1 x 6ml 20X Buffer 1 x 20ml Base Buffer 1 x 24ml Extraction Buffer 1 x 15ml LDH (Lactate Dehydrogenase) 1 x 187.5 units Microplate 96 antibody coated wells in 12 strips 1 unit PK (Pyruvate Kinase) 1 x 187.5 units Reagent Mix 1 x 0.2ml
- Research Areas
Our Abpromise guarantee covers the use of ab117994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ENO1 Human Activity Assay Kit images
Analysis of recombinant ENO1 (ab89248) and the neuron-specific recombinant ENO2 (ab78797), each at 2000 ng/mL. The relative ENO activity was determined from HepG2 standard curve as described in the protocol. The data show that the activity of the neuron-specific ENO2 can’t be detected.
Example of dilution series prepared from HepG2 cells extract.
Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994.
Scheme of reactions used in this kit. Abbreviations are as follows: enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).
References for ENO1 Human Activity Assay Kit (ab117994)
ab117994 has not yet been referenced specifically in any publications.