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|Sample type||Average %||Range|
|Cell culture supernatant||63||% - %|
|Serum||140||% - %|
ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.
|Components||1 x 96 tests|
|10X Blocking Buffer||1 x 6ml|
|20X Buffer||1 x 20ml|
|Base Buffer||1 x 24ml|
|Extraction Buffer||1 x 15ml|
|LDH (Lactate Dehydrogenase)||1 x 187.5 units|
|Microplate 96 antibody coated wells in 12 strips||1 unit|
|PK (Pyruvate Kinase)||1 x 187.5 units|
|Reagent Mix||1 x 1ml|
Our Abpromise guarantee covers the use of ab117994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|