ENO1 Human Activity Assay Kit (ab117994)

Overview

• Product nameENO1 Human Activity Assay Kit
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Cell extract	9			9%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Cell extract	4			11%

• Tests
  1 x 96 well plate
• Sample type
  Cell culture extracts, Tissue Extracts
• Assay typeEnzyme activity
• Sensitivity
  125 ng/ml
• Range
  125 ng/ml - 2000 ng/ml
• Recovery

  90 %
  

  Sample specific recovery

  Sample type	Average %	Range
  Cell culture supernatant	63	% - %
  Serum	140	% - %

• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Notes

  ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.

• Tested applicationsFunctional Studies more details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	1 x 96 tests
  10X Blocking Buffer	1 x 6ml
  20X Buffer	1 x 20ml
  Base Buffer	1 x 24ml
  Extraction Buffer	1 x 15ml
  LDH (Lactate Dehydrogenase)	1 x 187.5 units
  Microplate 96 antibody coated wells in 12 strips	1 unit
  PK (Pyruvate Kinase)	1 x 187.5 units
  Reagent Mix	1 x 0.2ml

• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Cancer Biomarker Panels
  • Biomarker Panels

  • Cancer
  • Tumor biomarkers
  • Enzymes
  • Other

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

ENO1 Human Activity Assay Kit images

• 

  Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
  Analysis of recombinant ENO1 (ab89248) and the neuron-specific recombinant ENO2 (ab78797), each at 2000 ng/mL. The relative ENO activity was determined from HepG2 standard curve as described in the protocol. The data show that the activity of the neuron-specific ENO2 can’t be detected.
• 

  Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
  Example of dilution series prepared from HepG2 cells extract.
• 

  Immunocytochemistry/ Immunofluorescence - ENO1 Human Activity Assay Kit (ab117994)
  Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994.
• 

  Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
  Scheme of reactions used in this kit. Abbreviations are as follows: enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).

Protocols

Protocol Booklet