Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Primary antibodies
- Proteins and Peptides
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
ENO1 Human Activity Assay Kit
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Cell extract | 9 | 9% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Cell extract | 4 | 11% |
Tests
1 x 96 well plate
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Enzyme activity
Sensitivity
125 ng/ml
Range
125 ng/ml - 2000 ng/ml
Recovery
90%
| Sample type | Average % | Range % |
|---|---|---|
| Cell culture supernatant | 63 | - |
| Serum | 140 | - |
Cross reactivity
Reacts with
Human
Notes
ab117994 is used to determine enolase 1 (ENO1) activity in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The enolase activity is determined via a coupled reaction to the consumption of NADH in an assay buffer.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at 4°C for upto 6 months. Refer to the protocol for further storage instructions.
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | P/N 8209802 | 1 x 6ml |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| Base Buffer | 1 x 24ml | |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
| LDH (Lactate Dehydrogenase) | 1 x 187.5 units | |
| Microplate strips | 1 unit | |
| PK (Pyruvate Kinase) | 1 x 187.5 units | |
| Reagent Mix | 1 x 0.2ml |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Cancer >> Cancer Biomarker Panels
Cancer >> Tumor biomarkers >> Enzymes >> Other
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Metabolism >> Energy Metabolism
Alternative names
- MS988
Applications
Show applications key
Our Abpromise guarantee covers the use of ab117994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
ENO1 Human Activity Assay Kit images:
Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
Analysis of recombinant ENO1 (ab89248) and the neuron-specific recombinant ENO2 (ab78797), each at 2000 ng/mL. The relative ENO activity was determined from HepG2 standard curve as described in the protocol. The data show that the activity of the neuron-specific ENO2 can’t be detected.
Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
Example of dilution series prepared from HepG2 cells extract.
Immunocytochemistry/ Immunofluorescence - ENO1 Human Activity Assay Kit (ab117994)
Immunofluorescent detection of ENO1 in cytosol in HeLa cells using ab112994.
Functional Studies - ENO1 Human Activity Assay Kit (ab117994)
Scheme of reactions used in this kit. Abbreviations are as follows: enolase (ENO), 2-phospho-D-glycerate (2PG), phosphoenolpyruvate (PEP), pyruvate kinase (PK), lactate dehydrogenase (LDH).
Protocols
References for ENO1 Human Activity Assay Kit (ab117994)
ab117994 has not yet been referenced specifically in any publications.
Publishing research using ab117994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"