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Read our guarantee »Overview
Product name
ENO1 Human ELISA Kit
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| HegG2 | 4 | 4.9% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| HegG2 | 4 | 4% |
Tests
1 x 96 well plate
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Sandwich
Sensitivity
219 ng/ml
Cross reactivity
Reacts with
Human
Product overview
ab116694 is an in vitro enzyme-linked immunosorbent assay to determine the levels of ENO1 protein in cell and tissue lysates. The assay employs a primary capture antibody specific for ENO1 coated on a 96-well plate. Samples and standards (available for purchase separately) are pipetted into the wells and ENO1 present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-ENO1 primary detection antibody is added. After washing away unbound detection antibody, HRP-conjugated detection antibody-specific secondary antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of ENO1 bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
Store all components at 4°C. The kits are stable for at least 6 months. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | P/N 8209802 | 1 x 6ml |
| 10X ENO1 Detector Antibody | 1 x 0.7ml | |
| 10X HRP Label | 1 x 1ml | |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
| Microplate 96 antibody coated wells in 12 strips | 1 unit | |
| TMB Development Solution | 1 x 6ml |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Cancer >> Cancer Biomarker Panels
Cancer >> Tumor biomarkers >> Other
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Metabolism >> Energy Metabolism
Immunology >> Immune System Diseases >> Allergens
Alternative names
- MS973
Applications
Show applications key
Our Abpromise guarantee covers the use of ab116694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Sandwich ELISA
sELISAsELISA
ENO1 Human ELISA Kit images:
ELISA - ENO1 Human ELISA Kit (ab116694)
Typical Standard Curve: Example ENO1 protein standard dilution series.
ELISA - ENO1 Human ELISA Kit (ab116694)
Typical Standard Curve: Example HeLa extract dilution series.
ELISA - ENO1 Human ELISA Kit (ab116694)
An experiment to determine the assay specificity. Analysis of recombinant ENO1 (ab89248) and the neuron-specific recombinant ENO2 (ab78797), each at 2000 ng/mL (shown in A), and of extracts prepared from human liver homogenate (HLH) and human heart homogenate (HHH), each at 200 ug/mL (shown in B) with the use of this kit.
ELISA - ENO1 Human ELISA Kit (ab116694)
Hypoxia-mimicking treatment by Deferoxamine increases ENO1 protein levels in HeLa cells. Analysis of extracts (100 ug/mL), prepared from HeLa cells treated for 24 hours with 0.5 mM Deferoxamine (DFO) or mock-treated (Vehicle), with the use of this kit.
Protocols
References for ENO1 Human ELISA Kit (ab116694)
ab116694 has not yet been referenced specifically in any publications.
Publishing research using ab116694? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"