![Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994) image](#ENO1-Primary-antibodies-ab112994-1.jpg)
![Flow Cytometry - ENO1 antibody [2G2AG11BF8] (ab112994) image](#ENO1-Primary-antibodies-ab112994-3.jpg)
![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994) image](#ENO1-Primary-antibodies-ab112994-4.jpg)
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Product name
Anti-ENO1 antibody [2G2AG11BF8]
See all ENO1 products (3) ...
Description
Mouse monoclonal [2G2AG11BF8] to ENO1
Specificity
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
Tested applications
IP, In-Cell ELISA, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Hela cell fraction
Positive control
HeLa and HL60 cells; HeLa, Human Liver and Heart homogenates.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab112994 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE.
Clonality
Monoclonal
Clone number
2G2AG11BF8
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Cofactors, Vitamins / minerals >> Co-factors
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Co-factors
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab112994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent concentration.
In-Cell ELISA: Use a concentration of 1 µg/ml.
Flow Cyt: Use a concentration of 1 µg/ml.
ICC/IF: Use a concentration of 5 µg/ml.
Target
Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similarities
Belongs to the enolase family.
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Post-translational
modifications
ISGylated.
Cellular localization
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
Target information above from: UniProt accessionP06733
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2 phospho D glycerate hydro lyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Alpha enolase antibody
see all
Database links
- Entrez Gene: 2023 Human
- Omim: 172430 Human
- SwissProt: P06733 Human
- Unigene: 517145 Human
Anti-ENO1 antibody [2G2AG11BF8] images:
Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
![Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)](/ps/datasheet/images/112/ab112994/ENO1-Primary-antibodies-ab112994-1.jpg)
Immunprecipitation of ENO1 by ab112994.
Staining of ENO1 (47 kDa) from HeLa cell lysate (1), Human liver (2) and Heart (3) homogenates. The identity of the protein was confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G.
Flow Cytometry - ENO1 antibody [2G2AG11BF8] (ab112994)
![Flow Cytometry - ENO1 antibody [2G2AG11BF8] (ab112994)](/ps/datasheet/images/112/ab112994/ENO1-Primary-antibodies-ab112994-3.jpg)
ab112994 at 1ug/ml staining ENO1 in MeOH-fixed HL60 cells by Flow Cytometry(blue). Isotype control antibody (red).
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)](/ps/datasheet/images/112/ab112994/ENO1-Primary-antibodies-ab112994-4.jpg)
ab112994 at 5ug/ml staining ENO1 in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly to the cytoplasm.
References for Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
ab112994 has not yet been referenced specifically in any publications.
Publishing research using ab112994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"