Overview
Product nameENO3 proteinSee all ENO3 proteins and peptides ...
Protein descriptionRecombinant full length Human ENO3 (amino acids 1-434) with an N terminal His tag (454 amino acids with the tag); predicted MWt: 49 kDa (Uniprot ID: P13929).
Uniprot accessionP13929
Molecular weight49kDa inclusive of tags
Protein length434 amino acids
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab113127 was purified using conventional chromatography.
Mass spectrometryMALDI-TOF
E.C. Number4.2.1.11
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 8.00
Constituents: 0.32% Tris HCl, 0.02% DTT, 20% Glycerol, 0.58% Sodium chloride
Concentration information loading...
SequenceMGSSHHHHHHSSGLVPRGSHMAMQKIFAREILDSRGNPTV EVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGK GVLKAVENINSTLGPALLQKKLSVADQEKVDKFMIELDGT ENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGN PDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE AMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILEN NEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLD FKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDD WATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCL LLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTF IADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK AIFAGRKFRNPKAK
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab113127
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
| MS |
MS: Use at an assay dependent concentration. |
Application notesThis peptide can be used with studies using ab157474.
Protein info
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Alternative names
2 phospho D glycerate hydrol yase2 phospho D glycerate hydrolyase2-phospho-D-glycerate hydro-lyase
Beta enolaseBeta-enolaseENO3ENOB_HUMANEnolase 3Enolase 3 (beta muscle)Enolase3GSD13MSEMuscle specific enolaseMuscle-specific enolaseSkeletal muscle enolase
see all
FunctionAppears to have a function in striated muscle development and regeneration.
Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Involvement in diseaseDefects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.
Sequence similaritiesBelongs to the enolase family.
Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Cellular localizationCytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
References for ENO3 protein (ab113127)
ab113127
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - ENO3 protein (ab113127)15% SDS-PAGE analysis of ENO3 protein (ab113127; 3 µg).