Anti-EPCR/CD201 antibody [M2] (ab56689)
Key features and details
- Mouse monoclonal [M2] to EPCR/CD201
- Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-EPCR/CD201 antibody [M2]
See all EPCR/CD201 primary antibodies -
Description
Mouse monoclonal [M2] to EPCR/CD201 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human EPCR/CD201 aa 1-250.
Database link: Q9UNN8 -
General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
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ICC/IF |
Use at an assay dependent concentration.
|
Notes |
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IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation. -
Tissue specificity
Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. -
Post-translational
modificationsN-glycosylated.
A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10544 Human
- Omim: 600646 Human
- SwissProt: Q9UNN8 Human
- Unigene: 647450 Human
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Alternative names
- Activated protein C receptor antibody
- APC receptor antibody
- APCR antibody
see all
Images
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Overlay histogram showing A549 cells stained with ab56689 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56689, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
This image was generated using the ascites version of the product.
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EPCR/CD201 antibody (ab56689) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human malignant lymphoma, diffuse large B tissue.
This image was generated using the ascites version of the product.
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Western blot against tagged recombinant protein immunogen using ab56689 EPCR/CD201 antibody at 1ug/ml. Predicted band size of immunogen is 52 kDa
This image was generated using the ascites version of the product.
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Immunofluorescence analysis of HeLa cells, staining EPCR/CD201 with ab56689 at 10 μ/ml.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab56689 has been referenced in 2 publications.
- Wang D et al. Protein C receptor is a therapeutic stem cell target in a distinct group of breast cancers. Cell Res 29:832-845 (2019). PubMed: 31481760
- Wei Y et al. Effect of cigarette smoke extract and nicotine on the expression of thrombomodulin and endothelial protein C receptor in cultured human umbilical vein endothelial cells. Mol Med Rep 17:1724-1730 (2018). PubMed: 29257196