Overview
- Product nameEPO Mouse ELISA kitSee all Erythropoietin (EPO) kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 15 pg/ml
- Range46.9 pg/ml - 3000 pg/ml
- Species reactivityReacts with: Mouse
- Product overview
ab119593 EPO Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse EPO species-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse EPO amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
- Notes
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well plate precoated with anti-mouse EPO antibody (12 strips) 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-mouse EPO antibody 1 x 130µl Lyophilized recombinant mouse EPO standard 2 x 10ng Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml -
Research Areas
Target information above from: UniProt accession
P01588
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- EPEPOEPO_HUMAN
- EpoetinErythropoietinMVCD2
see all
- Entrez Gene: 13856 Mouse
- SwissProt: P07321 Mouse
Applications
Our Abpromise guarantee covers the use of ab119593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
EPO Mouse ELISA kit images
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Sandwich ELISA - Erythropoietin (EPO) Mouse ELISA Kit (ab119593)Representative standard curve using ab119593.
Protocols
References for EPO Mouse ELISA kit (ab119593)
ab119593 has not yet been referenced specifically in any publications.
