EPO Receptor antibody [MM-0031-6G7] (ab56310)

Product Name 

EPO Receptor antibody [MM-0031-6G7]

Product type 

Primary antibodies

Description 

Mouse monoclonal [MM-0031-6G7] to EPO Receptor

Immunogen 

Human recombinant EPO Receptor (extracellular domain)

Reacts with 
(species key)

Hu

Specificity 

Ab56310 recognizes EPO Receptor using western blot. This antibody has been tested to be able to pick up human EPOR around 60-65 kDa under both reducing and non-reducing conditions. However the immunosignal is much stronger under non-reducing conditions so remove beta-mercaptoethanol or DTT from sample buffer.

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/50 - 1/300.
WB: 1/1000 - 1/2000. Use under non reducing conditions. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane; Single pass type I membrane protein. Isoform EPOR-S is secreted and located to the cell surface.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cardiovascular >> Atherosclerosis >> Ischemia / Reperfusion
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Immunology >> Immunoglobulins >> Receptors

Relevance 

The erythropoietin receptor (EPOR) is a member of the cytokine receptor family. There are several isoforms including: EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). Upon erythropoietin (EPO) binding, the EPOR activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to have a role in erythroid cell survival. Defects in the EPOR may produce erythroleukemia and familial erythrocytosis. A functional EPOR is found in the cardiovascular system, including endothelial cells and cardiomyocytes, and data suggest that the EPO/EPO receptor system plays an important role in cardiac function. In animal studies, treatment with EPO during ischemia/reperfusion in the heart has been shown to limit the infarct size and the extent of apoptosis.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MM-0031-6G7

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Lyophilised
Reconstitute with 0.5ml sterile PBS

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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