EPO Receptor antibody (ab10653)

Product Name 

EPO Receptor antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to EPO Receptor

Immunogen 

Recombinant full length protein (Human), expressed in mouse myeloma NSO cells.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit is approximately 0.3 ng/well.

WB: Use at a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 50 kDa. The detection limit is approximately 5 ng/lane under non-reducing and reducing conditions.

Endotoxin: < 10 ng/mg of antibody determined by the LAL method.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane; Single pass type I membrane protein. Isoform EPOR-S is secreted and located to the cell surface.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cardiovascular >> Atherosclerosis >> Ischemia / Reperfusion
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Immunology >> Immunoglobulins >> Receptors

Relevance 

The erythropoietin receptor (EPOR) is a member of the cytokine receptor family. There are several isoforms including: EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). Upon erythropoietin (EPO) binding, the EPOR activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to have a role in erythroid cell survival. Defects in the EPOR may produce erythroleukemia and familial erythrocytosis. A functional EPOR is found in the cardiovascular system, including endothelial cells and cardiomyocytes, and data suggest that the EPO/EPO receptor system plays an important role in cardiac function. In animal studies, treatment with EPO during ischemia/reperfusion in the heart has been shown to limit the infarct size and the extent of apoptosis.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for EPO Receptor antibody (ab10653)

  This product has been used in: (two most recent references)

Said HM et al. Expression patterns of the hypoxia-related genes osteopontin, CA9, erythropoietin, VEGF and HIF-1alpha in human glioma in vitro and in vivo. Radiother Oncol 83:398-405 (2007). PubMed: 17524506

Della Ragione F et al. Erythropoietin receptors on cancer cells: a still open question. J Clin Oncol 25:1812-3; author reply 1815 (2007). WB; Human. PubMed: 17470877
If you publish research using ab10653 please let us know so that we can cite the reference on this datasheet.

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