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Western blot - EPO Receptor antibody (ab61162)
Western blot - EPO Receptor antibody (ab61162)
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Product Name 

EPO Receptor antibody

 

See all EPO Receptor antibodies (7)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to EPO Receptor

Immunogen 

Synthetic non-phosphopeptide from human EPO Receptor, designed around the phosphorylation site of tyrosine 368 (DTYPLV)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Detects total EPO Receptor levels.

Tested applications 
(see key)

ELISA, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane; Single pass type I membrane protein. Isoform EPOR-S is secreted and located to the cell surface.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cardiovascular >> Atherosclerosis >> Ischemia / Reperfusion
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Immunology >> Immunoglobulins >> Receptors

Relevance 

The erythropoietin receptor (EPOR) is a member of the cytokine receptor family. There are several isoforms including: EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). Upon erythropoietin (EPO) binding, the EPOR activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to have a role in erythroid cell survival. Defects in the EPOR may produce erythroleukemia and familial erythrocytosis. A functional EPOR is found in the cardiovascular system, including endothelial cells and cardiomyocytes, and data suggest that the EPO/EPO receptor system plays an important role in cardiac function. In animal studies, treatment with EPO during ischemia/reperfusion in the heart has been shown to limit the infarct size and the extent of apoptosis.

 

EPO Receptor antibody (ab61162)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

2057    (Human)
13857    (Mouse)
24336    (Rat)

Omim

    

133171    (Human)

SwissProt

    

P19235    (Human)
P14753    (Mouse)
Q07303    (Rat)

Unigene

    

631624    (Human)
22394    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EPO Receptor antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for EPO Receptor antibody images, references, products related to ab61162 and other tools.

 

EPO Receptor antibody images:

Product image  Western blot - EPO Receptor antibody (ab61162)

Western blot - EPO Receptor antibody (ab61162)

All lanes : EPO Receptor antibody (ab61162) at 1/500 dilution

Lane 1 : Cos 7 cells treated with EPO (20U/ml, 15 mins)
Lane 2 : Cos 7 cells treated with EPO (20U/ml, 15 mins) with immunizing peptide


Predicted band size : 55 kDa
Observed band size : 55 kDa

 
 

Search PubMed (MEDLINE) for references to EPO Receptor

 

EPO Receptor antibody - more information

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EPO Receptor antibody - related products:

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