EPO Receptor antibody (ab61162)

Product Name 

EPO Receptor antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to EPO Receptor

Immunogen 

Synthetic non-phosphopeptide from human EPO Receptor, designed around the phosphorylation site of tyrosine 368 (DTY^PLV)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Detects total EPO Receptor levels.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane; Single pass type I membrane protein. Isoform EPOR-S is secreted and located to the cell surface.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Heart >> Apoptosis
Cardiovascular >> Atherosclerosis >> Ischemia / Reperfusion
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Immunology >> Immunoglobulins >> Receptors

Relevance 

The erythropoietin receptor (EPOR) is a member of the cytokine receptor family. There are several isoforms including: EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). Upon erythropoietin (EPO) binding, the EPOR activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated EPOR appears to have a role in erythroid cell survival. Defects in the EPOR may produce erythroleukemia and familial erythrocytosis. A functional EPOR is found in the cardiovascular system, including endothelial cells and cardiomyocytes, and data suggest that the EPO/EPO receptor system plays an important role in cardiac function. In animal studies, treatment with EPO during ischemia/reperfusion in the heart has been shown to limit the infarct size and the extent of apoptosis.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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