![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EPO antibody [Epo2] (ab20375) image](#EPO-Primary-antibodies-ab20375-1.jpg)
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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Overview
Product name
Anti-EPO antibody [Epo2]
See all EPO products (12) ...
Description
Mouse monoclonal [Epo2] to EPO
Tested applications
IHC-P, ELISA, WB, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
Epo2
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Hematopoietic system development
Cardiovascular >> Blood >> Erythrocytes
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Signal Transduction >> Growth Factors/Hormones >> Hormones
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EPO antibody [Epo2] (ab20375)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EPO antibody [Epo2] (ab20375) image](/ps/datasheet/images/20/ab20375/EPO-Primary-antibodies-ab20375-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab20375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 21 kDa.
sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [Epo1] to EPO (ab110656).
Target
Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Cellular localization
Secreted
Alternative names
- Erythropoietin precursor antibody
- EP antibody
- EP antibody
see all
Database links
- Entrez Gene: 2056 Human
- Omim: 133170 Human
- SwissProt: P01588 Human
- Unigene: 2303 Human
Anti-EPO antibody [Epo2] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EPO antibody [Epo2] (ab20375)
IHC image of ab20375 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20375, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-EPO antibody [Epo2] (ab20375)
ab20375 has not yet been referenced specifically in any publications.
Publishing research using ab20375? Please let us know so that we can cite the reference in this datasheet
![EPO antibody [Epo2] for WB in Human (20375)](/ps/Reviews/Images/ab10189_52505.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"