Anti-EPO antibody (ab226956)
Key features and details
- Rabbit polyclonal to EPO
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-EPO antibody
See all EPO primary antibodies -
Description
Rabbit polyclonal to EPO -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment within Human EPO (internal sequence). The exact sequence is proprietary.
Database link: P01588 -
Positive control
- IHC-P: Rat liver tissue. WB: Human liver tissue extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/1000.
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WB |
1/5000 - 1/20000.
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ELISA |
1/1000 - 1/10000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/5000 - 1/20000. |
ELISA
1/1000 - 1/10000. |
Target
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Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 2056 Human
- Entrez Gene: 24335 Rat
- Omim: 133170 Human
- SwissProt: P01588 Human
- SwissProt: P29676 Rat
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Alternative names
- EP antibody
- EPO alpha antibody
- EPO antibody
see all
Images
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Anti-EPO antibody (ab226956) at 1/10000 dilution + recombinant human EPO protein (with glycosylation) expressed in CHO cells at 0.1 µg
12% SDS-PAGE gel.
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Paraffin-embedded rat liver tissue stained for EPO using ab226956 at 1/400 dilution in immunohistochemical analysis.
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Anti-EPO antibody (ab226956) at 1/500 dilution + Human liver tissue extract at 30 µg
12% SDS-PAGE gel.
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An ELISA plate is coated with 50 μL of human EPO recombinant protein at concentration ranged from 0.125 μg/mL to 4 μg/mL. The coated protein is detected with EPO antibody at 1 μg/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab226956 has been referenced in 3 publications.
- Liu F et al. Electroacupuncture Improves Cerebral Ischemic Injury by Enhancing the EPO-JAK2-STAT5 Pathway in Rats. Neuropsychiatr Dis Treat 17:2489-2498 (2021). PubMed: 34354356
- Kuloglu N et al. Investigation of vascular endothelial growth factor receptor-dependent neuroplasticity on rat nucleus tractus solitarius and phrenic nerve after chronic sustained hypoxia. J Biochem Mol Toxicol 35:e22918 (2021). PubMed: 34541741
- Samson FP et al. Dual Switch Mechanism of Erythropoietin as an Antiapoptotic and Pro-Angiogenic Determinant in the Retina. ACS Omega 5:21113-21126 (2020). PubMed: 32875248