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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Overview
Product name
Anti-EPO antibody
See all EPO products (12) ...
Description
Rabbit polyclonal to EPO
Specificity
No crossreactivity to a cytokine test panel (ELISA).
Tested applications
ELISA, WB, Neutralising, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
CHO cell derived recombinant human EPO.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 40% Glycerol, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Hematopoietic system development
Cardiovascular >> Blood >> Erythrocytes
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Signal Transduction >> Growth Factors/Hormones >> Hormones
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.5 - 1 µg/ml.(Detection limit is ~1ng/well.)
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 21 kDa.
Neut: Use a concentration of 1 - 3 µg/ml.(1 hour at 37°C. The suggested antibody concentration can detect 0.3 units/ml recombinant human EPO in cell line TF1.)
IHC-P: Use a concentration of 15 µg/ml
Target
Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Cellular localization
Secreted
Alternative names
- Erythropoietin precursor antibody
- EP antibody
- EPO alpha antibody
see all
Database links
- Entrez Gene: 2056 Human
- Omim: 133170 Human
- SwissProt: P01588 Human
- Unigene: 2303 Human
References for Anti-EPO antibody (ab30545)
This product has been referenced in:
- Rades Det al. Prognostic impact of erythropoietin expression and erythropoietin receptor expression on locoregional control and survival of patients irradiated for stage II/III non-small-cell lung cancer. Int J Radiat Oncol Biol Phys 80:499-505 (2011). IHC-P; Human.Read more (PubMed: 20646855) »
- Medana IMet al. Erythropoietin and its receptors in the brainstem of adults with fatal falciparum malaria. Malar J 8:261 (2009). IHC-P; Human.Read more (PubMed: 19930602) »
See all 2 publications for this product
Publishing research using ab30545? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"