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Overview

Product name

Anti-EPO antibody
See all EPO products (12) ...

Description

Rabbit polyclonal to EPO

Specificity

No crossreactivity to a cytokine test panel (ELISA).

Tested applications

ELISA, WB, Neutralising, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

CHO cell derived recombinant human EPO.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 40% Glycerol, PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab30545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    ELISA

     ELISA: Use a concentrati...Read more →

    ELISA: Use a concentration of 0.5 - 1 µg/ml.(Detection limit is ~1ng/well.)

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 21 kDa.

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    Neutralising

     Neut: Use a concentratio...Read more →

    Neut: Use a concentration of 1 - 3 µg/ml.(1 hour at 37°C. The suggested antibody concentration can detect 0.3 units/ml recombinant human EPO in cell line TF1.)

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    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 15 µg/ml

Target

Relevance

Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Cellular localization

Secreted

Alternative names

  • Erythropoietin precursor antibody
  • EP antibody
  • EPO alpha antibody
  • EPO antibody
  • Epoetin antibody
  • Erythropoetin antibody
  • Erythropoietin antibody
  • MGC138142 antibody
see all

References for Anti-EPO antibody (ab30545)

This product has been referenced in:

  • Rades Det al. Prognostic impact of erythropoietin expression and erythropoietin receptor expression on locoregional control and survival of patients irradiated for stage II/III non-small-cell lung cancer. Int J Radiat Oncol Biol Phys 80:499-505 (2011). IHC-P; Human.Read more (PubMed: 20646855) »
  • Medana IMet al. Erythropoietin and its receptors in the brainstem of adults with fatal falciparum malaria. Malar J 8:261 (2009). IHC-P; Human.Read more (PubMed: 19930602) »

See all 2 publications for this product

Publishing research using ab30545? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"