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Product Name
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EPO antibody
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See all EPO antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to EPO
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Immunogen
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CHO cell derived recombinant human EPO.
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Reacts with
(species key)
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Hu
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Specificity
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No crossreactivity to a cytokine test panel (ELISA).
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Tested applications
(see key)
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ELISA, IHC-P, Neut, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Use at a concentration of 0.5 - 1 µg/ml. Note: detection limit is ~1ng/well.
IHC-P: Use at a concentration of 15 µg/ml.
Neut: Use at a concentration of 1 - 3 µg/ml for 1 hour at 37ºC. Note: The suggested antibody concentration can detect 0.3 units/ml recombinant human EPO in cell line TF1.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia Developmental Biology >> Organogenesis >> Hematopoietic system development Cardiovascular >> Blood >> Erythrocytes Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules Signal Transduction >> Growth Factors/Hormones >> Hormones Cardiovascular >> Angiogenesis >> Growth Factors >> Other
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Relevance
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Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None Constituents: 40% Glycerol, PBS, pH 7.2 Material safety datasheet (MSDS) for this product: Glycerol MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Notes
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this EPO antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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References for EPO antibody (ab30545)
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This product has been used in:
Medana IM et al. Erythropoietin and its receptors in the brainstem of adults with fatal falciparum malaria. Malar J 8:261 (2009). IHC-P; Human. PubMed: 19930602
If you publish research using ab30545 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to EPO
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EPO antibody - more information
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Customer reviews (feedback) regarding EPO antibody |
Customer FAQs regarding EPO antibody |
Protocols for EPO antibody |
Price and availability of products related to EPO antibody |
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Contact Abcam with a Technical Enquiry about EPO antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"