EPO antibody (ab30545)

Product Name 

EPO antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to EPO

Immunogen 

CHO cell derived recombinant human EPO.

Reacts with 
(species key)

Hu

Specificity 

No crossreactivity to a cytokine test panel (ELISA).

Tested applications 
(see key)

ELISA, IHC-P, Neut, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.5 - 1 µg/ml.
Note: detection limit is ~1ng/well.

IHC-P: Use at a concentration of 15 µg/ml.

Neut: Use at a concentration of 1 - 3 µg/ml for 1 hour at 37ºC.
Note: The suggested antibody concentration can detect 0.3 units/ml recombinant human EPO in cell line TF1.

WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Hematopoietic system development
Cardiovascular >> Blood >> Erythrocytes
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Signal Transduction >> Growth Factors/Hormones >> Hormones
Cardiovascular >> Angiogenesis >> Growth Factors >> Other

Relevance 

Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 40% Glycerol, PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

References for EPO antibody (ab30545)

  This product has been used in:

Medana IM et al. Erythropoietin and its receptors in the brainstem of adults with fatal falciparum malaria. Malar J 8:261 (2009). IHC-P; Human. PubMed: 19930602

If you publish research using ab30545 please let us know so that we can cite the reference on this datasheet.

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