Recombinant Anti-EPS8 antibody [EPR6112] (ab124882)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6112] to EPS8
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EPS8 antibody [EPR6112]
See all EPS8 primary antibodies -
Description
Rabbit monoclonal [EPR6112] to EPS8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human EPS8 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- HeLa, A375, 293T, and K562 cell lysates, Human kidney tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6112 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommended. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 92 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. antigen retrieval is recommended. |
Target
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Function
Upon binding to EGF receptor enhances EGF-dependent mitogenic signals. Can bind multiple cellular targets. -
Tissue specificity
Expressed in all tissues analyzed, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Expressed in all epithelial and fibroblastic lines examined and in some, but not all, hematopoietic cells. -
Sequence similarities
Belongs to the EPS8 family.
Contains 1 PH domain.
Contains 1 SH3 domain. -
Domain
The SH3 domain mediates interaction with SHB. -
Post-translational
modificationsPhosphorylated by several receptor tyrosine kinases. - Information by UniProt
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Database links
- Entrez Gene: 2059 Human
- Omim: 600206 Human
- SwissProt: Q12929 Human
- Unigene: 591160 Human
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Alternative names
- Epidermal growth factor receptor kinase substrate 8 antibody
- Epidermal growth factor receptor pathway substrate 8 antibody
- EPS 8 antibody
see all
Images
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All lanes : Anti-EPS8 antibody [EPR6112] (ab124882) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa -
Staining of EPS8 in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124882 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124882 has been referenced in 2 publications.
- Lu H et al. Identification of HLA-A*1101-restricted cytotoxic T lymphocyte epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep 14:4999-5006 (2016). WB ; Human . PubMed: 27840923
- Tang B et al. Identification of human leukemia antigen A*0201-restricted epitopes derived from epidermal growth factor pathway substrate number 8. Mol Med Rep 12:1741-52 (2015). PubMed: 25936538